Method for improving human in-vitro fertilization embryo development rate

An in vitro fertilization and development rate technology, applied in the field of regenerative medicine, can solve problems affecting the normal development and quality of embryos, changes in gene expression, etc., and achieve the effect of promoting cell division and proliferation and improving development rate.

Inactive Publication Date: 2018-10-30
DALIAN MEDICAL UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

During this process, changes in the external environment will lead to changes in gene expression, thereby affecting the normal development and quality of embryos.

Method used

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  • Method for improving human in-vitro fertilization embryo development rate
  • Method for improving human in-vitro fertilization embryo development rate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of human oocyte fertilization fluid in vitro containing traditional Chinese medicine monomer baicalin

[0022] According to the three mass ratios (volume ratios) of baicalin and human oocyte conventional culture medium: 4.5ug / mL, 6.0ug / mL, and 7.5ug / mL, add conventional culture medium, and set the volume to 100mL, corresponding to the test group (Bai1, Bai2, Bai3), after mixing, use a 0.22 μm microporous membrane (Millipore) to filter and store in a 4°C refrigerator for later use.

Embodiment 2

[0023] Example 2 Preparation of Human Oocyte In vitro Semen Containing Chinese Medicine Monomer Icariin

[0024] According to the three mass ratios (volume ratios) of icariin (Ica) and human oocyte conventional culture medium: 1.25ug / mL, 2.0ug / mL, and 3.0ug / mL, the corresponding test groups are (Ica1, Ica2 , Ica3), the preparation of mixed fertilized fluid, the specific embodiment refers to Example 1.

Embodiment 3

[0025] Example 3 Preparation of Human Oocyte In vitro Semen Containing Chinese Medicine Monomer Ligustrazine

[0026] According to the three mass ratios (volume ratios) of ligustrazine (Lig) and human oocyte conventional culture medium: 2.0ug / mL, 3.0ug / mL, 4.0ug / mL, the corresponding test groups are (Lig1, Lig2, Lig3 ) to prepare mixed fertilized fluid, the specific implementation is referring to Example 1.

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Abstract

The invention provides a method for improving the human in-vitro fertilization embryo development rate. The method is characterized in that an in-vitro fertilization culture solution is prepared by adding a traditional Chinese medicine monomer in a human oocyte conventional culture solution to improve the human in-vitro fertilization embryo development rate, wherein the traditional Chinese medicine monomer is any of baicalin, icariin and ligustrazine, and the mass ratio (or volume ratio) range of the traditional Chinese medicine monomer and the human oocyte conventional culture solution is 6.0ug/mL, 2.0 ug/mL and 3.0 ug/mL. The method provided by the invention has the benefits that studies show that the fertilization culture solution prepared by adding the baicalin as the traditional Chinese medicine monomer in the conventional culture solution has a good promoting effect on human in-vitro fertilization embryo development and is better than a control group without a traditional Chinese medicine ingredient in aspects of cleavage rate, blastocyst development rate and the like.

Description

technical field [0001] The invention belongs to the field of regenerative medicine, and in particular relates to a method for improving the development rate of human in vitro fertilized embryos. Background technique [0002] Fertilization-embryo transfer (IVF-ET), also known as "test-tube baby", is a technique in which sperm and eggs from the body are developed into fertilized eggs in a test tube, and then transplanted back to the mother's uterus to achieve the purpose of conception. It is the main means of human assisted reproductive technology. After more than 30 years of development, IVF technology has enabled the maturation, fertilization and embryonic development of mammalian oocytes to be studied more intuitively in vitro, and has been widely used to obtain healthy offspring for infertility patients made a great contribution. [0003] The famous "fetal origin hypothesis" in the field of human medicine believes that assisted reproductive technology has solved many hum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
CPCC12N5/0604C12N2500/30
Inventor 孔英隋琳琳孔慧隋雨新
Owner DALIAN MEDICAL UNIVERSITY
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