Serum-free adipose-derived stem cell culture medium and culture method of adipose-derived stem cells (ADSCs)

A technique for adipose stem cells and a culture method, which is applied in the field of serum-free adipose stem cell culture medium and adipose stem cell culture, can solve the problems of insufficient growth-promoting components, damage to the proliferation activity of adipose stem cells, and difficulty in obtaining the number of cells, so as to promote growth and Proliferation ability, reducing proliferative activity damage, and improving the effect of in vitro amplification efficiency
CN110373384APending Publication Date: 2019-10-25安徽科门生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
安徽科门生物科技有限公司
Publication Date
2019-10-25

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Abstract

The invention discloses a serum-free adipose-derived stem cell culture medium and a culture method of adipose-derived stem cells (ADSCs). The culture medium comprises a basic culture medium body and additives, the basic culture base body is a DMEM (dulbecco's modified eagle medium) / F12, the additives include transferrin, fibroblasts growth factors, C-algocyan, acanthopanax senticosus saponins B, scabiolide-3-O-glucosides and beta-ecdysterone. By the arrangement, the growth and proliferation of the ADSCs can be promoted, the damage of proliferation activity of the ADSCs during culture can be reduced, and thus, the in-vitro amplification efficiency of the ADSCs is greatly improved.
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Description

Technical field

[0001] The invention relates to the field of biotechnology, in particular to a serum-free adipose stem cell culture medium and a method for culturing adipose stem cells. Background technique

[0002] Adipose-derived stem cells (ADSCs) are adult stem cells extracted from adipose tissue, which have the potential to differentiate into cartilage, bone, fat and other tissues. Adipose tissue is easily obtained through liposuction or surgery, is less traumatic to the donor and does not involve ethical issues. Moreover, adipose stem cells are relatively widely distributed in humans and animals, and the expansion operation is simple and convenient. They are one of the most potential seed cells in the fields of medicine and tissue engineering.

[0003] At present, the in vitro culture of adipose stem cells mainly uses a medium containing fetal bovine serum. Due to the presence of animal-derived serum components, there is a potential safety hazard to cause rejection, and the ...

Claims

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