Mesenchymal stem cell (MSC) derivative liquid and preparation method thereof as well as application to cosmetics

A technology for stem cells and derivative solutions, applied in the field of mesenchymal stem cell derivative solutions and their preparation, can solve the problem that the living environment and metabolism of epidermal cells cannot be fundamentally improved, that they are not suitable for sensitive skin or pregnant women, and that macromolecular substances are not easy to skin. Absorption and other problems, to achieve long-term moisturizing effect, improve splitting proliferation, improve the effect of redness

Active Publication Date: 2015-12-23
诺赛联合(北京)生物医学科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Since the beginning of the 21st century, with the continuous development of the country, the continuous progress of the society, the improvement of people's living standards and the pursuit of their own beauty, people, especially women, pay more and more attention to daily skin care and beauty. However, the cosmetic products on the market are mixed with good and bad. , it is difficult for consumers to identify
[0003] Many fine chemical skin care products contain substances harmful to human skin such as lead and mercury, ethanol, colorants and mineral oil. When consumers use these skin care products every day, they unknowingly cause certain damage to their skin. Can cause dermatitis and infection problems
Moreover, most of the cosmetics sold in the existing market are processed through the extraction of plant components or fine chemical production processes. The macromolecular substances in the products are not easily absorbed by the skin, causing an additional burden on the skin.
In addition, due to technical bottlenecks and production capacity, cosmetic products at the current stage are basically not biologically active, and cannot fundamentally improve the living environment and metabolism of epidermal cells, and have no repairing effect on skin damage caused by external factors, especially not suitable for sensitive skin. Pregnant women use

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  • Mesenchymal stem cell (MSC) derivative liquid and preparation method thereof as well as application to cosmetics
  • Mesenchymal stem cell (MSC) derivative liquid and preparation method thereof as well as application to cosmetics

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Experimental program
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Effect test

Embodiment 1

[0029] A method for preparing a mesenchymal stem cell-derived liquid, comprising the following steps:

[0030] (1) The mesenchymal stem cells derived from human placental adipose tissue were primary cultured and subcultured to the seventh generation using the mesenchymal stem cell culture medium, and the cell culture medium of the seventh generation was collected;

[0031](2) Add 1-3 propylene glycol, sodium hyaluronate, royal jelly, stem cell growth factor, antagonizing melanin growth factor, vascular endothelial growth factor, glutathione, trehalose and chitosan oligosaccharide to the cell culture medium obtained in step (1). Sugar, so that the concentration of 1-3 propylene glycol is 100g / L, the concentration of sodium hyaluronate is 8mg / L, the concentration of royal jelly is 5mg / L, the concentration of stem cell growth factor is 1.5mg / L, and the concentration of antagonizing melanin growth factor The concentration of vascular endothelial growth factor is 4mg / L, the concent...

Embodiment 2

[0033] (1) Using the mesenchymal stem cell medium to culture the mesenchymal stem cells derived from human placental adipose tissue, pass to the third generation after primary culture, and collect the third generation cell culture medium;

[0034] (2) add 1-3 propanediol, sodium hyaluronate, royal jelly, growth factor, glutathione, trehalose and chitosan oligosaccharide in step (1) gained cell culture medium, make the concentration of 1-3 propanediol be 50g / L, the concentration of sodium hyaluronate is 10mg / L, the concentration of royal jelly is 3mg / L, the concentration of stem cell growth factor is 2mg / L, the concentration of antagonizing melanin growth factor is 3mg / L, and the concentration of vascular endothelial growth factor is 3 mg / L, the concentration of glutathione is 2 mg / L, the concentration of trehalose is 6 mg / L, and the concentration of chitosan oligosaccharide is 2 mg / L to obtain the mesenchymal stem cell-derived liquid.

Embodiment 3

[0036] (1) The mesenchymal stem cells derived from human placental adipose tissue were primary cultured and subcultured to the seventh generation using the mesenchymal stem cell culture medium, and the cell culture medium of the seventh generation was collected;

[0037] (2) Add 1-3 propanediol, sodium hyaluronate, royal jelly to step (1) gained cell culture medium, make the concentration of 1-3 propanediol be 80g / L, the concentration of sodium hyaluronate be 5mg / L, royal jelly The concentration is 6mg / L, and the mesenchymal stem cell-derived liquid is obtained.

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Abstract

The invention discloses mesenchymal stem cell (MSC) derivative liquid and a preparation method thereof as well as application to cosmetics. The preparation method of the MSC derivative liquid comprises the following steps of (1) culturing MSCs derived from placental adipose tissue by using an MSC culture medium, and collecting cell culture fluid; (2) adding 1 to 3 parts of propylene glycol, sodium hyaluronate and bee milk into the obtained cell culture fluid in step 1 so as to obtain the MSC derivative liquid. The obtained MSC derivative liquid is used for the cosmetics, and is good in moisturizing effect, capable of reducing fine wrinkles, improving skin smoothness, improving skin elasticity, alleviating conditions of skin redness, decrustation and inflammation, and reducing the occurrence of skin allergy.

Description

technical field [0001] The invention relates to a skin care product and its preparation method and application, in particular to a mesenchymal stem cell derived liquid, its preparation method and its application in cosmetics. Background technique [0002] Since the beginning of the 21st century, with the continuous development of the country, the continuous progress of the society, the improvement of people's living standards and the pursuit of their own beauty, people, especially women, pay more and more attention to daily skin care and beauty. However, the cosmetic products on the market are mixed with good and bad. , difficult for consumers to identify. [0003] Many fine chemical skin care products contain substances harmful to human skin such as lead and mercury, ethanol, colorants and mineral oil. When consumers use these skin care products every day, they unknowingly cause certain damage to their skin. Can cause dermatitis and infection problems. Moreover, the cosme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/60A61K8/64A61Q19/00A61Q19/08
CPCA61K8/60A61K8/64A61K8/987A61K8/988A61Q19/00A61Q19/08
Inventor 亓爱杰
Owner 诺赛联合(北京)生物医学科技有限公司
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