3D dynamic input type tissue reaction device

A reaction device and input technology, which is applied in tissue cell/virus culture devices, biochemical instruments, biochemical equipment and methods, etc., can solve the problems of cell feeding and other problems, and achieve the effect of not easy to pollute and easy to use

Inactive Publication Date: 2004-11-03
卢建熙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the above-mentioned existing technologies can provide a three-dimensional culture environment for cells/tissues in tissue eng

Method used

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  • 3D dynamic input type tissue reaction device
  • 3D dynamic input type tissue reaction device

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1, select a cubic cell / tissue culture box 1 with a size of 100×70×60 mm. The porous biological support body 5 in the incubator is made of tricalcium phosphate, has a microstructure with a porosity of 75%, a pore diameter of 500 to 600 microns and an internal connection diameter of 80 to 120 microns, and is a cylinder with a diameter of 20 mm and a length of 50 mm. The diameter of the central channel is 2.2 mm. Other compositions and structures of the present invention are as described above and will not be repeated here.

[0032] Prepare bone marrow stem cells (10 6 Cells / ml) suspension 16ml is injected into the biological support body 5 through the three-way valve 47; Connect microcirculation pump 42 simultaneously, make it in working condition, form microcirculation, flow rate is 10ml per hour. The liquid was replaced once a week; after two weeks of cultivation, it could be seen that a large number of cells formed and accumulated in the pores of the biologi...

Embodiment 2

[0033]In Example 2, a hemispherical cell / tissue culture box (not shown) with a diameter of 100 mm was selected. The porous biological support body 5 in the incubator 1 is made of tricalcium phosphate, has a microstructure with a porosity of 75%, a pore diameter of 500 to 600 microns and an internal connection diameter of 80 to 120 microns, and is a long cube of 20×20×60 mm in size. , the diameter of the central channel is 2mm. Other compositions and structures of the present invention are as described above and will not be repeated here.

[0034] Preparation of articular chondrocytes (10 7 Cell / ml) suspension 25ml is injected into the biological support body 5 through the three-way valve 47, and after 4 hours of storage, inject 200ml of the cell culture solution with calf serum through the three-way valve 47. Connect microcirculation pump 42 simultaneously, make it in working condition, form microcirculation, flow rate is 10ml per hour. The liquid was replaced once a week; ...

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Abstract

The dynamic 3D input tissue reactor includes one culture case for inoculating cell onto biological rack with central channel; at least one canal stretched into the central channel of the biological rack, and one circular infusion device communicated with the canal. The culture case has one inlet port and one outlet port, and via the inlet port the canal is communicated with the circular infusion device. Via the closed circular infusion device, suspended cell liquid is fed directly to the central position of biological rack material inside the culture case, and after the cell attached to wall of the biological rack, micro cell culturing liquid is fed continuously with micro circulating pump to provide cell with enough nutrients for cell division and proliferation to form new tissue.

Description

technical field [0001] The present invention relates to a tissue reaction device used in the fields of biology and medicine, especially a dynamic three-dimensional input tissue reactor used in tissue reconstruction engineering in vitro. Background technique [0002] Recently, with the development of life science, material science and related physical and chemical disciplines, a new technical field has emerged, that is, biological tissue engineering, which is the application of cell biology and engineering principles to research and develop repair and improve damage. A science of biological substitutes for tissue structure and function. The basic method is to adsorb normal tissue cells cultured and expanded in vitro on a biological scaffold with good biocompatibility that can be degraded and absorbed by the body, and implant it into the tissue defect site of the body. During the gradual degradation of the scaffold material, the cells continue to proliferate. And secrete the ...

Claims

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Application Information

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IPC IPC(8): C12M3/00
Inventor 卢建熙戴克戎M.戴斯凯帕斯P.哈道恩K.恩赛尔姆汤亭亭
Owner 卢建熙
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