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Benthic diatom culture method

A technology of benthic diatoms and culture methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve the problems of limited density, many impurities, and easy introduction of harmful organisms, etc., to achieve operational Simple, suitable for popularization and application, and low-cost effects

Inactive Publication Date: 2018-05-08
SHANDONG ORIENTAL OCEAN SCI TECH
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AI Technical Summary

Problems solved by technology

Due to the specificity of the lifestyle of benthic diatoms, suspension static culture cannot be carried out like planktonic microalgae
At present, the benthic diatom baits used in production are generally natural mixed benthic diatoms collected from large seaweeds in local sea areas, and are directly thrown into culture ponds with attachment substrates for subculture, resulting in a lot of impurities, Limited density and easy introduction of predators or other competing algae or even toxic microalgae

Method used

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Embodiment Construction

[0016] 1. Separation, purification and cultivation of algal species:

[0017] The mixed algae species of benthic diatoms were obtained from the sea area of ​​Yunxi base of Shandong Oriental Ocean, and the impurities such as bacteria were removed by centrifugation for many times, and the improved F medium was used for static enrichment culture in a petri dish. After 7-10 days, the benthic diatoms are attached to the glass slide at the bottom of the culture dish, the growth conditions are observed under a microscope, and the monoclonal cells of the benthic diatoms are separated by micropipette method or solid plate method.

[0018] The benthic diatom species obtained through separation and purification were placed in 500mL Erlenmeyer flasks respectively, and the improved F medium was used to boil seawater normally, with a salinity of 31 and a light intensity of 50μmolE·m -2 the s -1 , the photoperiod is 16L:8D. The temperature is 20°C. (see attached figure 1 )

[0019] 2. E...

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Abstract

The invention belongs to the industry of aquaculture and particularly relates to a benthic diatom culture method used for culturing breeding feed. The method is characterized by comprising the following steps: inoculating navicula tenera obtained by separation and purification and nitzchia palea to a liquid F culture medium in certain cell density, putting a circulating pump in the liquid F culture medium, and performing intermittent circulation, namely performing circulation for 10 minutes at intervals of every 2 hours, wherein the formula of the improved F culture medium comprises 149.6mg ofNaNO3, 8.8mg of NaH2PO4, 3.9mg of FeC6H5O7.5H2O, 1mL of trace element solution mother liquor, 1mL of vitamin solution mother liquor, 1000mL of pure water, vitamin mother liquor, 100mg of vitamin B1,0.5mg of vitamin B12 and 500mL of pure water; culturing benthic diatom in a 5000mL conical flask; and normally boiling seawater by adopting the improved F culture medium, wherein the salinity is 31, the illumination intensity is 50mu mol E.m<-2>s<-1>, the illumination period is 16L:8D, and the temperature is 20 DEG C. The benthic diatom culture method is simple, convenient and fast in operation, low in cost and suitable for popularization and application.

Description

technical field [0001] The invention belongs to the aquaculture industry, in particular to a method for cultivating benthic diatoms used for cultivating aquaculture feed. Background technique [0002] Marine benthic diatoms are important bait in the production of rare aquatic animal seedlings such as abalone, sea cucumber and sea urchin. Cultivating sufficient high-quality benthic diatoms is one of the important links for the successful production of artificial seedlings. Due to the specificity of life style of benthic diatoms, it is impossible to carry out suspension static culture like planktonic microalgae. At present, the benthic diatom baits used in production are generally natural mixed benthic diatoms collected from large seaweeds in local sea areas, and are directly thrown into culture ponds with attachment substrates for subculture, resulting in a lot of impurities, Density is limited and easy to introduce predators or other competing algae and even toxic microalga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 陈书秀张壮志孙娟李霞刘学迁潘金华赵楠王青岩李晓捷
Owner SHANDONG ORIENTAL OCEAN SCI TECH
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