A method for rapid identification of ivory-participating sea cucumbers
A rough sea cucumber and ivory technology, applied in the field of species identification, can solve the problems of feature disappearance, difficulty in identifying sea cucumber species, and morphological destruction
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[0027] 1. Collection of sea cucumber specimens: M1-dried ivory sea cucumber; M2-dried rough sea cucumber; M3-collected sea cucumber samples from random markets
[0028] 2. Pretreatment of test samples: first step, first replace the alcohol in the sea cucumber alcohol-preserved samples and soak the dried sea cucumber samples. The solutions used are PBS (137mmol / L NaCl, 2.7mmol / L KCl, 10mmol / L Na2HPO4 , 2mmol / LKH2PO4, pH 7.2) In the second step, take 50-80mg of the sample processed in the previous step and put it in 800-1000μl high-salt buffer (200mmol / L Tris-HCl, 50mmol / L EDTA, 500mmol / L NaCl, 0.2 % β-mercaptoethanol, pH 8.0), centrifuge at 10000-12000g / min for 1-2min, discard the supernatant; repeat the operation twice. Proteinase K was incubated at 65°C for 1 hour until the tissue was completely dissolved. Add an equal volume of chloroform:isoamyl alcohol (24:1) and mix well, centrifuge at 15,000×g for 10 minutes at room temperature, and carefully pipette the supernatant int...
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