A kind of dna barcode standard detection gene of catfish plateau loach and its application

A standard detection and barcode technology, which is applied in the direction of recombinant DNA technology, DNA / RNA fragments, microbial measurement / inspection, etc., can solve the problems of no gene sequence and no data related to catfish plateau loach, and achieve the effect of shortening the identification time

Active Publication Date: 2018-11-20
深圳华大海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the endemism of many fishes in our country, there is not enough relevant data
There is also no related gene sequence

Method used

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  • A kind of dna barcode standard detection gene of catfish plateau loach and its application
  • A kind of dna barcode standard detection gene of catfish plateau loach and its application
  • A kind of dna barcode standard detection gene of catfish plateau loach and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Obtaining of the Standard Sequence of Pseudomonas plateau loach

[0033] 1. Morphological identification and sample collection and storage of Pseudomonas plateau loach

[0034]According to the plateau loach, the catfish has a thick body, a broad front end, slightly flattened, a nearly round rear end, and a thin round tail peduncle. Head large, flat, triangular in back view. Mouth large, inferior, arc-shaped. Lips without mastoids, mandible spoon-shaped. Small eyes. The body has no scales, and the skin on the body surface is scattered with short strips and papillary cortical protrusions. The dorsal side of the body is yellowish brown, the abdomen is light yellow, the back and sides of the body have dark brown circles and cloud spots, and all fins have spots and other morphological characteristics, as well as the joint identification of relevant fish taxonomy experts. . Muscle tissue was cut and stored in 95% ethanol in a -20°C refrigerator.

[0035] 2. D...

Embodiment 2

[0051] 1. Collection, preservation and processing of samples to be tested

[0052] Cut part of the muscle tissue of the sample to be tested from the soaked sample, store it in 95% alcohol and store it in a -20°C refrigerator.

[0053] 2. DNA extraction

[0054] The DNA in the samples was extracted by the phenol-chloroform method and stored at -20°C for future use.

[0055] 3. PCR primer synthesis

[0056] This method selects the sequence of 2 pairs of primers as follows:

[0057] FishF2_t1:5'-TGTAAAACGACGGCCAGTCGACTAATCATAAAGATATCGGCAC-3' (SEQ ID NO.2)

[0058] VF2_t1:5'-TGTAAAACGACGGCCAGTCAACCAACCACAAAGACATTGGCAC-3' (SEQ ID NO.3)

[0059] FishR2_t1:5'-CAGGAAACAGCTATGACACTTCAGGGTGACCGAAGAATCAGAA-3' (SEQ ID NO.4)

[0060] FR1d_t1:5'-CAGGAAACAGCTATGACACCTCAGGGTGTCCGAARAAYCARAA-3' (SEQ ID NO.5)

[0061] 4. PCR reaction system (25ul)

[0062]

[0063]

[0064] 5. PCR reaction procedure

[0065]

[0066] After the reaction is over, turn on the PCR instrument, take ...

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PUM

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Abstract

The invention discloses a DNA (Deoxyribose Nucleic Acid) bar code standard detection gene of triphophysa siluroides and application of the DNA bar code standard detection gene. The DNA bar code standard detection gene is a COI gene, with the nucleotide sequence shown as SEQ ID NO.1. A DNA bar code species identification method of the triphophysa siluroides adopts a reliable DNA molecular identification technology and the triphophysa siluroides is rapidly and accurately identified. Compared with a traditional morphological identification method, a standard gene sequence obtained by the method is good for molecular identification of the triphophysa siluroides and identification time can be effectively shortened.

Description

technical field [0001] The invention belongs to the field of animal molecular biology, and in particular relates to a DNA barcode standard detection gene of catfish plateau loach and its application. Background technique [0002] Triplophysa siluroides (scientific name: Triplophysa siluroides) is a fish belonging to the genus Platelophysa Cyprinidae of the order Cyprinidae, which is endemic to China. It is a plateau river fish, distributed in the main and tributaries of the upper reaches of the Yellow River and affiliated lakes, etc., and more common in the estuaries of the upper reaches of the main and tributaries and affiliated lakes. Changxi lurks in the gravel bottom, also inhabits alluvial silt, slow flow and still water with many aquatic plants, and lives as a bottom dweller. The type locality of this species is in the upper reaches of the Yellow River. The fish body is stout, with a broad front end, slightly flattened, a nearly round rear end, and a thin round tail ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888
Inventor 朱志飞章晋勇石琼马兴宇王敏吴菲菲
Owner 深圳华大海洋科技有限公司
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