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A standard gene sequence of safflower dna barcode

A gene sequence and barcode technology, applied in the field of specific primer combination, can solve the problem of difficult to distinguish appearance, and shorten the identification time.

Active Publication Date: 2018-06-26
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For multi-component agricultural products and traditional Chinese medicines, after processing, it is usually difficult to distinguish by appearance

Method used

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  • A standard gene sequence of safflower dna barcode
  • A standard gene sequence of safflower dna barcode
  • A standard gene sequence of safflower dna barcode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Utilize HHF1651 / HHR1822 primer combination, wherein

[0045] HHF1651 such as SEQ ID NO.2 in the sequence listing;

[0046] HHR1822 such as SEQ ID NO.3 in the sequence listing;

[0047] Perform PCR amplification identification.

[0048] 1. Experimental materials

[0049] 1.1 Plant material

[0050] The different safflower varieties tested are shown in Table 1.

[0051] Table 1 The name and origin of safflower varieties

[0052]

[0053] The other 17 different species of materials are: sunflower (Helianthus annuus), soybean (Glycinemax), corn (Zea mays), cotton (Gossypium hirsutum), tomato (Solanum lycopersicum), wheat (Triticum), barley (Hordeum vulgare) , cowpea (Vigna unguiculata), carrot (Daucus carota), rape (Brassica napus), pepper (Capsicum annuum), peanut (Arachis hypogaea), sesame (Sesamum indicum), radish (Raphanus sativus), rice (Oryza sativa), tobacco ( Nicotianatabacum), Arabidopsis thaliana.

[0054] 1.2 Enzymes and Reagents

[0055] Molecular bio...

Embodiment 2

[0083] Utilize HHF1651 / HHR1822 primer combination, wherein

[0084] HHF1651 such as SEQ ID NO.2 in the sequence listing;

[0085] HHR1822 such as SEQ ID NO.3 in the sequence listing;

[0086] Perform PCR amplification identification.

[0087] 1. Experimental materials

[0088] 1.1 Plant material

[0089] Sunflower petals, safflower petals, cakes containing safflower ingredients, rapeseed cakes, safflower pollen, rapeseed pollen.

[0090] 1.2 Enzymes and Reagents

[0091] Molecular biology reagents, TAKARA Taq DNA polymerase, 10×PCR buffer, dNTP Mixture (10mM), MgCl 2 purchased from Dalian Bao Biological Company. PCR primers were synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd.

[0092] 1.3 Experimental Instruments

[0093] PCR instrument: DNA Engine Dyad Peltier Thermal Cycler (Bio-Rad, USA);

[0094] Nucleic acid electrophoresis instrument: DYYIII type YY0115-94 (Beijing Liuyi Instrument Factory);

[0095] Gel imaging system: Gel Doc XR Sys...

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Abstract

The invention provides a safflower DNA bar code standard detection gene sequence and detection primer combination. The safflower DNA bar code standard detection gene sequence is characterized in that the bar code standard detection gene sequence is shown in the gene sequence SEQ ID NO.1. The gene is obtained after amplification through a polymerase chain reaction, the result is determined according to the detected sample amplification product, and if a band 171bp strip can be obtained after amplification in a specific manner, the situation that the detected sample contains the safflower component can be determined. The invention provides a method for specific detection of a safflower component. The method is helpful for identification of the safflower component and adulteration is prevented. When the method is compared with traditional morphological identification method, the obtained gene sequence facilitates to achieve safflower molecular identification and safflower identification time can be shortened effectively.

Description

technical field [0001] The invention relates to the field of species identification, in particular to a safflower species-specific gene sequence and a specific primer combination based on the gene sequence for specifically detecting safflower components. Background technique [0002] Safflower (scientific name: Carthamus tinctorius, English name: Safflower) is an annual or biennial herb of the genus Safflower in the family Asteraceae. When it comes to safflower, it is easy for people to confuse safflower with saffron. Saffron (also known as saffron, saffron) is a plant of the genus Crocus (Crocus) in the family Iridaceae, and there is an essential difference between the two. Safflower is originally produced in Egypt, India, and the Mediterranean coast. It is wild and cultivated in the Soviet Union. It is widely cultivated in Korea and Japan. Now it is more produced in more than ten countries such as India, Canada, the United States, and Australia. Safflower is a foreign coun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/11C12Q1/6895
Inventor 覃瑞张丽柯杰刘虹李刚邱秀双王龙体
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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