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Saponin antimycotic compounds in holothuria scabra jaeger and preparation method thereof

A compound and saponin technology, which is applied in the field of saponin antifungal compounds, can solve the problems such as antifungal activity of saponin compounds that have not been seen

Inactive Publication Date: 2008-05-28
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But there is no report on the extraction and isolation of saponins scabraside A and scabraside B from rough sea cucumber and their antifungal activity

Method used

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  • Saponin antimycotic compounds in holothuria scabra jaeger and preparation method thereof
  • Saponin antimycotic compounds in holothuria scabra jaeger and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1: Preparation of scabraside A and scabraside B

[0034]Take 3000 grams of dry rough sea cucumber grown in the South China Sea of ​​Hainan, China, grind it, and extract it with twice the weight of 55%-60% ethanol successively under reflux for 5 times, each extraction for 1 hour. The ethanol extracts were combined, and the ethanol was recovered under reduced pressure to obtain a liquid extract. The liquid extract was dissolved in water of 3 times the weight, filtered, and the filtrate was passed through a macroporous resin column HP60 of Mitsubishi Corporation of Japan, and eluted with 5000 ml of water, 50% and 70% ethanol in sequence. Collect 50% and 70% ethanol eluate, recover the solvent under reduced pressure to dryness, and obtain 70 grams of total saponins.

[0035] The total saponins were subjected to silica gel column chromatography, eluted with about 1500 ml of chloroform:methanol:water=8:2:1 mixed solvent (lower layer), collected the part containing sa...

Embodiment 2

[0036] Embodiment 2: Preparation of scabraside A and scabraside B

[0037] Take 2000 grams of dry sea cucumber grown in the South China Sea of ​​Hainan, China, grind it and extract it according to the method described in Example 1 to obtain 41 grams of total saponins. The total saponins were subjected to silica gel column chromatography, eluted with about 1000 ml of chloroform:methanol:water=6.5:3.5:1 (lower layer), collected the part containing saponins, and then carried out C18 reverse phase column chromatography, and 59% methanol / Eluted with water, collected fractions containing scabraside A and scabraside B respectively, recovered to dryness under reduced pressure to obtain 0.066 g of scabraside A and 0.12 g of scabraside B.

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Abstract

The invention relates to medicine technical field, in particular to two saponin antimycotics compounds as scabraside A and scabraside B, which molecular formulas are represented as C54H85O26SNa and C54H85O27SNa, separated from sea cucumber. The chemical structure and spatial configuration of the compounds are assured by kinds of modern spectrographic analysis, particularly by kinds of advanced two-dimension nuclear magnetic resonance spectroscopy. The compounds can effectively restrain various bacterial strains as candida albicans and aspergillus fumigatus by external antimycotics test. The inventive compounds can be used to prepare antimycotics drug. The invention provides lead compound for preparing new antimycotics drug, with significant value for developing and utilizing sea medical biological resource.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to saponin antifungal compounds scabraside A and scabraside B isolated from marine animal sea cucumber and a preparation method thereof. Background technique [0002] Coarse sea cucumber (Holothuria scabra Jaeger) is a species of Holothuria scabra Jaeger that grows in large numbers in the central and western parts of Guangdong, my country, Dongxing, Guangxi, and Hainan Island. It belongs to the genus Holothuria scabra. The body length is generally 30-40cm, the diameter is 8-10cm, the body wall is thick, the bone fragments are abundant, and the edges are smooth. Rough sea cucumber is one of the important edible sea cucumbers. It is big and thick, and has excellent quality. Because the flesh is white and transparent, it is also called Mingyu ginseng, white ginseng or sand ginseng. It has high medicinal value and is a nourishing treasure [edited by Liao Yulin, Chinese Animal History, ...

Claims

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Application Information

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IPC IPC(8): C07J71/00A61K31/7048A61P31/10A61K35/56A61K35/616
Inventor 易杨华韩华李玲刘宝姝
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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