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A natural fluorescent dye obtained from a marine invertebrate, compositions from a marine invertebrate, compositions containing the said dye and their use

A fluorescent dye and composition technology, applied in the field of extraction, purification and identification of this new dye, can solve the problem of no report of marine animals

Inactive Publication Date: 2003-05-21
科学和工业研究委员会
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0029] Few reports of marine animals

Method used

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  • A natural fluorescent dye obtained from a marine invertebrate, compositions from a marine invertebrate, compositions containing the said dye and their use
  • A natural fluorescent dye obtained from a marine invertebrate, compositions from a marine invertebrate, compositions containing the said dye and their use
  • A natural fluorescent dye obtained from a marine invertebrate, compositions from a marine invertebrate, compositions containing the said dye and their use

Examples

Experimental program
Comparison scheme
Effect test

example 2

[0245] Animals were collected from the west and central coast of India at low tide. Bring it to the laboratory, carry out taxonomic identification and keep it in a glass jar filled with seawater with a salinity of 30-32 / par (30%) before further use. Animals are adults and sexually mature. Example 2 extracting pigment

[0246] Tried 2 methods:

[0247] 1) In our initial experiments, animals were frozen at -20°C after collection, and when thawed, some of the pigment went into the tray. Carrying out this operation, according to this method, the pigment can be extracted 3-4 times from the same animal. Figures 1 and 2 show fresh animals and animals that underwent 4 extractions.

[0248] 2) In the 2nd method, wash the animal first with tap water, then with ultrapure water (MQ water). Cut the animal body with sharp shears, and separate the body wall from the internal organs. Peel the skin from the body wall with a sharp razor and store in a -20 °C freezer if not immediately fur...

example 4

[0251] The colored solution was decanted and filtered by means of a peristaltic pump with a glass filter of a microfiltration unit (Vensil make). The filtrate was placed on an orbital shaker and shaken at 200 rpm for half an hour. Example 4 concentrated pigment

example 5

[0252] Then the colored solution was placed on a water bath at 80°C, and concentrated to 1 / 3 of its volume, and the ethanol was also evaporated at the same time. The concentrate was filtered again with the same filter. Example 5 Purification of Dye

[0253] The pigment solution will contain impurities such as NaCl, MgCl 2 , MgSO 4 and other water-soluble compounds. If a polar organic solvent such as ethanol (dehydrated), acetone is added to a concentrated solution of the pigment, it can be seen that the pigment will precipitate rapidly.

[0254] This allows the pigment to be separated from the sea salt. The pigment is thus purified for spectrophotometric analysis.

[0255] The concentrated solution prepared in Example 5 above was placed in a 500 ml separatory funnel for separation, and ethanol (80 ml concentrated supernatant + 100 ml 99.5% ethanol) was added thereto. Tilt the separatory funnel to gently mix the contents and collect the precipitate overnight. The precipi...

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Abstract

The present invention discloses the process of extraction, purification and characterization of a fluorescent dye from a marine echinoderm Holothuria scabra, compositions containing the dye and various applications of the dye.

Description

Technical field [0001] The present invention relates to a novel fluorescent dye obtained from the marine invertebrate, Holothuria scabra. The present invention also provides a method for extracting, purifying and identifying the new dye, which is a natural dye obtained from marine invertebrates, especially sea cucumbers. Sea cucumbers are echinoderms, members of the group Echinodermata, which also includes starfish and sea urchins. They have the following taxonomic positions. Background technique [0002] Said sea cucumber has the following taxonomic position: [0003] Subkingdom: Metazoa [0004] Phylum: Echinodermata Echinodermata [0005] Subphylum: Eleutherozoa [0006] Class: sea cucumber class [0007] Subclass: Aspidochirotacea, [0008] Dendrochirotacea, [0009] Apodacea [0010] Order: Dendrochirota, [0011] Shield hand (As pidochirota), [0012] ...

Claims

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Application Information

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IPC IPC(8): C12Q1/02A01N63/00A61K8/00A61K8/96A61K8/98A61K35/56A61P33/00A61P35/00A61Q19/00C07G99/00C09B61/00C09B67/54C09D7/12C09D11/00C09D201/00C09K11/06
Inventor 乌沙·戈斯瓦米阿努托什·甘古利
Owner 科学和工业研究委员会
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