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Super-resolution fluorescent digit holographic sectioning microimaging system and method

A super-resolution and digital holography technology, which is applied in the fields of fluorescence/phosphorescence, analytical materials, material excitation analysis, etc., can solve the problems of large data volume, large amount of numerical calculation, and inappropriateness, and achieve fast acquisition speed and super-resolution The effect of high-rate optical tomography

Active Publication Date: 2017-06-23
BEIJING UNIV OF TECH
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Problems solved by technology

However, the scanning process of this method takes a long time, the amount of recorded data is large, and the amount of numerical calculation involved is large and time-consuming
Therefore, it is not suitable for dynamic sample imaging or observation of dynamic processes in the sample

Method used

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  • Super-resolution fluorescent digit holographic sectioning microimaging system and method
  • Super-resolution fluorescent digit holographic sectioning microimaging system and method
  • Super-resolution fluorescent digit holographic sectioning microimaging system and method

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Embodiment 1

[0031] In the computer, numerical simulation software is used to simulate the imaging process of the super-resolution fluorescence digital holographic tomographic microscopic imaging system of the present invention and the subsequent numerical processing method. The super-resolution optical tomographic fluorescent digital holographic microscopic imaging system adopted is shown in figure 1 , select as attached figure 2 Two samples with different structures are shown, and they are placed one after the other at different depths in the sample 11 space. Simulate the imaging process of the system, after recording all the holograms required, the results of reconstruction using the reconstruction algorithm are attached image 3 (b) shown. Contrast attached image 3 The results obtained using a conventional fluorescence microscopy imaging system given in (a), it can be seen that the crosstalk between two object images at different depths in the sample space image 3 (b) is smaller;...

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Abstract

The invention relates to a super-resolution fluorescent digit holographic sectioning microimaging system and method and belongs to the field of fluorescent digit holographic and super-resolution imaging. Laser after amplitude modulation through a spatial light modulator loading a proper mask structure enters a microobjective to generate exciting light in a three-dimensional structure on a to-be-image sample; excited fluorescence of the sample is collected by the microobjective, and is then diffracted and divided and shifted in phase through the spatial light modulator through a dichroscope and an imaging system, and the two beans of light intervene in a position where an image detector is located to form a hologram which is recorded; the recorded hologram is reconstructed through a numerical algorithm in a computer, and for a structure in certain depth in the sample, reconstructed images of super-resolution and optical section can be separately acquired; the reconstructed images of super-resolution and optical section are fused by means of the numerical algorithm, so that super-resolution optical sectioning imaging is realized; and the reproducing distance of the hologram is changed, so that three-dimensional imaging of the super-resolution optical sections of structures in different depths of the sample can be realized.

Description

technical field [0001] The invention discloses a super-resolution fluorescent digital holographic three-dimensional tomographic imaging system and method based on structured illumination and fluorescent digital holography, belonging to the technical field of fluorescent digital holography and super-resolution imaging. Background technique [0002] Digital holography, as an interferometric imaging technique, enables three-dimensional numerical reconstruction or quantitative phase-contrast imaging of coherently illuminated sample images. Digital holographic microscopy has been widely used in specific research in the field of biomedicine. In actual imaging applications, compared with traditional microscopic imaging techniques, digital holographic microscopy has the advantages of fast, real-time, three-dimensional and high precision. The emergence of fluorescent digital holography has solved the dependence of traditional digital holography on the coherence of illumination sourc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6458
Inventor 满天龙万玉红周宏强韩影
Owner BEIJING UNIV OF TECH
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