The invention discloses a method for extracting and separating gelsevirine, koumidine, koumine, gelsemine and
furan koumine from
gelsemium elegans benth. The method comprises the following steps: extracting and neutralizing
gelsemium elegans benth raw materials by using an acidic water solution, and
drying under reduced pressure, thus obtaining gelsemic
acid water extract; extracting the gelsemic
acid water extract by using an
organic solvent, concentrating, dissolving by water and carrying out
freeze drying, thus obtaining
gelsemium elegans benth extract
powder; separating and purifying the
gelsemium elegans benth
powder, thus respectively obtaining the gelsevirine, the koumidine, the koumine, the gelsemine and the
furan koumine. According to the method disclosed by the invention, the gelsevirine, the koumidine, the koumine, the gelsemine and the
furan koumine which are high in purity can be extracted and separated from the
gelsemium elegans benth, the operation is simple, the cost is low, simultaneous separation of various alkaloids in the
gelsemium elegans benth is realized, and the method is beneficial for comprehensive utilization of natural resources.