Method for determining content of pyrroloquinoline quinone in biological sample by magnetic solid-phase extraction-capillary zone electrophoresis
A biological sample and capillary technology, applied in the field of biological analysis, can solve the problems of cumbersome steps, heavy workload, and expensive C18 column, and achieve the effect of good symmetry and improved separation efficiency
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[0032] Example 1
[0033] (1) Capillary electrophoresis analysis conditions:
[0034] Uncoated quartz capillary column (41.5cm×50μm, Hebei Yongnian Ruifeng Chromatography Device Co., Ltd.), the new capillary is rinsed with methanol for 10min, distilled water for 5min, 1mol / L NaOH solution for 30min, distilled water for 5min, and finally Rinse running buffer for 10-15 minutes. The running buffer and sample solution were filtered with a 0.22μm microporous needle filter. Before each injection, rinse with distilled water and running buffer for 2 minutes. The detection wavelength is 249nm, the sampling method is pressure injection (0.5psi, 10.0 seconds), the separation voltage is 25kV, and the capillary column temperature is 25°C. The running buffer is: 10mmol / L borax-10mmol / L β-cyclodextrin mixture, pH=8.8, containing trifluoroacetic acid with a mass concentration of 0.1%.
[0035] (2) Selection of buffer system
[0036] The buffer system and concentration directly affect ion migratio...
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