Flow type fluorescence microscopy imaging device and method

A microscopic imaging and flow fluorescence technology, which is applied in the direction of measuring devices, fluorescence/phosphorescence, instruments, etc., can solve the problems of affecting the real-time performance of the system, imaging tailing of moving objects, and taking up time, etc.
CN103308440AInactive Publication Date: 2013-09-18香港浸会大学深圳研究院
0 Cites 19 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
香港浸会大学深圳研究院
Publication Date
2013-09-18
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a flow type fluorescence microscopy imaging device, in order to solve the problems of small focal depth of an imaging flow cytometer as well as trailing which occurs when a moving object is imaged. The imaging device has the following beneficial effects that (1) auto-fluorescence or non-auto-fluorescence of cells is excited by a light sheet source with the thickness near a diffraction limit, so that the problem of small focal depth of fluorescence microscopy imaging can be solved; (2) only the cells positioned within the range of focal depth are excited to emit fluorescence and a background of a fluorescence image can be lowered effectively, so that the sensitivity of a system can be enhanced effectively; (3) the cells perpendicularly pass through an excitation light source and are subjected to fluorescence imaging in a way that the imaging direction is parallel to the flowing direction of a sample, so that the trailing phenomenon can be well inhibited; (4) multiple cells are allowed to be detected simultaneously, so that the detection speed of the system can be increased.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present application relates to a flow fluorescence microscopy imaging device, more specifically to a fluorescence microscopy imaging device combining single-layer light-excited fluorescence microscopy and flow cytometry. Background technique

[0002] In the fields of biology and medicine, flow cytometry is the main detection tool when large numbers of cells need to be scanned. Traditional flow cytometers observe cells as points, and can obtain high detection speed without completely sacrificing spatial resolution. Currently, its detection speed can reach tens of thousands of cells per second. Imaging flow cytometry can not only obtain the "point" information obtained by traditional flow cytometry, but also perform microscopic imaging of cells to obtain spatial information of cells. Compared with traditional flow cytometers, imaging flow cytometers have greater advantages when it is necessary to obtain information about the morphology and internal...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More