Identification method for Tibetan herb Saibei Corydalis

An identification method and a technology for the medicinal material of Corydalis are applied in the field of identification of the Tibetan medicinal material Corydalis saibei, which can solve the problems of backward quality standards of Corydalis saibei, inability to accurately and effectively identify Corydalis saibei, lack of content determination indicators, etc. , The identification method is simple and the effect of ensuring the accuracy

Active Publication Date: 2014-11-05
SHANDONG JINHE DRUG RES DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is that the current quality standard of Corydalis sylvestris is relatively backward, and there are only traits and thin-layer identification items without any content measurement index, so it is impossible to accurately and effectively identify Corydalis sylvestris and control the quality of finished medicines. Provide a quality detection method of Tibetan medicinal material Corydalis cypress

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  • Identification method for Tibetan herb Saibei Corydalis
  • Identification method for Tibetan herb Saibei Corydalis
  • Identification method for Tibetan herb Saibei Corydalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] The Tibetan medicinal material Corydalis Saibei was provided by Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.

[0085] Determination of the content of total alkaloids:

[0086] Take about 10g of the fine powder of this product, accurately weigh it, put it into a stoppered conical flask, add 50ml of chloroform-ammonia water mixed solution with a volume ratio of 100:1, weigh it, ultrasonic for 30min, and place it overnight (12 hours) , filtered, the residue was added to 50ml of chloroform-ammonia mixed solution with a volume ratio of 100:1, shaken for 1 hour, filtered, and the residue was washed 3 times with a chloroform-ammonia mixed solution with a volume ratio of 100:1, each time 15m, filter; combine the above filtrate and lotion, evaporate to dryness at low temperature and reduced pressure (below 40°C), add 10ml of ethanol to the residue to dissolve, add 1g of activated carbon, shake for 10min to decolorize, filter, and accurately measure 5ml of subsequent f...

Embodiment 2

[0099] The Tibetan medicinal material Corydalis Saibei was provided by Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.

[0100] Determination of the content of total alkaloids:

[0101] Take about 8g of the medicinal powder of this product, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 50ml of chloroform-ammonia water mixed solution with a volume ratio of 100:1, weigh it, ultrasonic for 30min, let stand overnight, filter, Add 40ml of chloroform-ammonia water mixed solution with volume ratio of 100:1 to the residue, shake for 1 hour, filter, wash residue with 100:1 volume of chloroform-ammonia water mixed solution for 3 times, each time 15m, filter Combine the above filtrate and lotion, evaporate to dryness at low temperature and reduced pressure (below 40°C), add 10ml of ethanol to the residue to dissolve, add 1g of activated carbon, shake for 10min to decolorize, filter, and accurately measure 5ml of the filtrate to the Erlenmeyer flask. Accurately...

Embodiment 3

[0114] The Tibetan medicinal material Corydalis Saibei was provided by Qinghai Jinhe Tibetan Medicine Pharmaceutical Co., Ltd.

[0115] Determination of the content of total alkaloids:

[0116] Take about 12g of the medicinal material fine powder of this product, accurately weigh it, put it in a stoppered conical flask, add 50ml of chloroform-ammonia water mixed solution with a volume ratio of 100:1, weigh it, ultrasonic for 30min, let stand overnight, filter, Add 60ml of chloroform-ammonia water mixed solution with a ratio of 100:1 by volume to the residue, shake for 1 hour, filter, wash the residue with 100:1 chloroform-ammonia mixed solution for 3 times, each time 15m, filter Combine the above filtrate and lotion, evaporate to dryness at low temperature and reduced pressure (below 40°C), add 10ml of ethanol to the residue to dissolve, add 1g of activated carbon, shake for 10min to decolorize, filter, and accurately measure 5ml of the filtrate to the Erlenmeyer flask. Accur...

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Abstract

The invention provides an identification method for a Tibetan herb Saibei Corydalis. The identification method comprises the following steps: preparing a reference sample, preparing a test sample, and acquiring a fingerprint map of the Saibei Corydalis through high-performance liquid chromatography, wherein the chromatographic conditions and the system suitability test are as follows: octadecylsilane bonded silica is used as a filler; acetonitrile is used as a mobile phase A, an aqueous solution containing 0.2% glacial acetic acid and 0.5% triethylamine in percentage by volume is used as a mobile phase B, the pH value of the mobile phase B is 6.0, and gradient elution is performed; the detection wavelength is 285nm; counted by an original protopine peak, the number of theoretical plates is not be less than 3000. The identification method for the Saibei Corydalis is simple and convenient, and high in reproducibility and accuracy; as long as the selected herb has the fingerprint map specified in the application, the herb is confirmed as the Saibei Corydalis, therefore, the accuracy of herb selection is ensured and further the quality of a finished medicine is further ensured.

Description

technical field [0001] The invention relates to an identification method of a medicinal material, in particular to an identification method of a Tibetan medicinal material Corydalis sergei. Background technique [0002] There are about 320 species of Corydalis in the world, mainly produced in Asia. There are about 200 species in my country, and they are distributed all over the country. Corydalis saibei is the dried whole plant of Corydalis saibeiensis (also known as Corydalis saibeiensis), which is distributed in Qinghai, Tibet, Gansu, Sichuan and other places. It is a commonly used drug in Tibetan medicine, and the data shows that 25% It is used in the treatment of various hemorrhage, hepatitis, cholecystitis, influenza and other diseases. It is also used to treat bruises or other trauma, fever, gastroenteritis and so on. [0003] Judging from the current research results on the chemical components of this genus, this genus mainly contains prototropines, protoberberines, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/02
Inventor 孙绪丁李怀平赵艳霞杨文钰
Owner SHANDONG JINHE DRUG RES DEV
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