High-purity wild blueberry anthocyanin industrial purification system and purification method

Abstract

The high-purity wild blueberry anthocyanin industrial purification system and its purification method belong to the technical field of blueberry anthocyanin processing. The tubular storage tank is arranged horizontally, and the tubular storage tank is connected with the inlets of multiple macroporous resin columns, and the low-purity horizontal tank and the high-purity horizontal tank are respectively connected with the outlets of all macroporous resin columns through pipelines. A method for industrial purification of high-purity wild blueberry anthocyanins, comprising the following steps: S1. washing the macroporous resin column with purified water; S2. absorbing the concentrate by the macroporous resin column; S3. simultaneously washing the inside of the macroporous resin column with pure water; S4. Detect the polysaccharide content of the water in the liquid discharged from the macroporous resin column; S5. The release solution that is equipped with 20% ethanol enters multiple macroporous resin columns at the same time for release; S6. injects the release solution of 60% ethanol; S7 .Purified water for resin flushing. The separation and purification system of the invention reduces the cost and improves the separation and purification efficiency. The separation and purification method of the present invention can obtain high-purity anthocyanins by using a gradient analysis method.

Description

technical field [0001] The invention belongs to the technical field of blueberry anthocyanin processing, in particular to a high-purity wild blueberry anthocyanin industrial purification system and a purification method thereof. Background technique [0002] The current problems encountered in the industrial production of the separation and purification of wild blueberry anthocyanins at home and abroad: 1). The purity of anthocyanins after separation and purification is relatively low. At present, the highest purity in the world is generally 36% HPLC, and impurities are not greatly removed. , with similar polarity and molecular weight to anthocyanins, there are many substances with similar molecular weight, so the purity is low, which affects its activity and application range; 2). The separation and purification system is primitive and cannot realize on-line monitoring, continuous, automation, production, etc.; 3 ). The separation and purification method is single, and the ...

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Problems solved by technology

[0002] The current problems encountered in the industrial production of the separation and purification of wild blueberry anthocyanins at home and abroad: 1). The purity of anthocyanins after separation and purification is relatively low. At present, the highest purity in the world is generally 36% HPLC, and impurities are not greatly removed. , with similar polarity and molecular weight to anthocyanins, there are many substances with similar molecular weight, so the purity is low, which affects its activity and application range; 2). The separation and purification system is primitive and cannot realize on-line monitoring, continuous, automation, production, etc.; 3 ). The separation and purification method is single, and the optimal system and elution solvent ratio are not used; 4). During the separation and purification process, chromatographic tailing is prone to occur, resulting in uneven separation and impurities; 5). Macroporous adsorption resin elution Solvent residues and anthocyanin residues are not clean; 6). The life cycle of macroporous adsorption resin is short, and the elution cost is high; 7). Manual operation is cumbersome, the method is primitive, and the production cost is high;

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