High-purity wild blueberry anthocyanin industrial purification system and purification method thereof

Abstract

The invention discloses a high-purity wild blueberry anthocyanin industrial purification system and a purification method thereof, and belongs to the technical field of blueberry anthocyanin processing. A tubular storage tank is horizontally arranged, the tubular storage tank is communicated with inlets of a plurality of macroporous resin columns, and a low-purity horizontal tank and a high-purityhorizontal tank are respectively communicated with outlets of all the macroporous resin columns through pipelines. The industrial purification method of high-purity wild blueberry anthocyanin comprises the following steps of: S1, flushing the macroporous resin columns with purified water; S2, adsorbing a concentrated solution by using a macroporous resin column; S3, flushing the interior of the macroporous resin columns with pure water at the same time; S4, detecting the polysaccharide content of water in the liquid discharged from the macroporous resin column; S5, allowing a desorption solution with 20% ethanol to simultaneously enter the plurality of macroporous resin columns for desorption; S6, injecting a desorption solution of 60% ethanol; and S7, flushing the resin with purified water. According to the separation and purification system, the cost is reduced, and the separation and purification efficiency is improved. The separation and purification system provided by the invention reduces the cost and improves the separation and purification efficiency. The separation and purification method disclosed by the invention utilizes gradient analysis to acquire high-purity anthocyanin.

Description

technical field [0001] The invention belongs to the technical field of blueberry anthocyanin processing, in particular to a high-purity wild blueberry anthocyanin industrial purification system and a purification method thereof. Background technique [0002] The current problems encountered in the industrial production of the separation and purification of wild blueberry anthocyanins at home and abroad: 1). The purity of anthocyanins after separation and purification is relatively low. At present, the highest purity in the world is generally 36% HPLC, and impurities are not greatly removed. , with similar polarity and molecular weight to anthocyanins, there are many substances with similar molecular weight, so the purity is low, which affects its activity and application range; 2). The separation and purification system is primitive and cannot realize on-line monitoring, continuous, automation, production, etc.; 3 ). The separation and purification method is single, and the ...

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Problems solved by technology

[0002] The current problems encountered in the industrial production of the separation and purification of wild blueberry anthocyanins at home and abroad: 1). The purity of anthocyanins after separation and purification is relatively low. At present, the highest purity in the world is generally 36% HPLC, and impurities are not greatly removed. , with similar polarity and molecular weight to anthocyanins, there are many substances with similar molecular weight, so the purity is low, which affects its activity and application range; 2). The separation and purification system is primitive and cannot realize on-line monitoring, continuous, automation, production, etc.; 3 ). The separation and purification method is single, and the optimal system and elution solvent ratio are not used; 4). During the separation and purification process, chromatographic tailing is prone to occur, resulting in uneven separation and impurities; 5). Macroporous adsorption resin elution Solvent residues and anthocyanin residues are not clean; 6). The life cycle of macroporous adsorption resin is short, and the elution cost is high; 7). Manual operation is cumbersome, the method is primitive, and the production cost is high;

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