Liquid chromatography column and high performance liquid chromatography analytical method

A liquid chromatographic column and chromatographic column technology, which is applied in the field of new liquid chromatographic column and high performance liquid chromatographic analysis, can solve problems such as chromatographic peak tailing, achieve the effect of improving resolution and broad application prospects

CN101393187BInactive Publication Date: 2012-05-09BEIJING UNIV OF CHEM TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2012-05-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a novel liquid phase chromatographic column. The chromatographic column comprises a chromatographic column tube, chromatographic column heads arranged on two ends of the chromatographic column, a gasket, a sieve plate and a column core; the diameter of the column core is less than the inside diameter of the chromatographic column tube; the column core and the chromatographic column tube are coaxially arranged in the center of the chromatographic column; materials are filled into the annular section between the column core and the chromatographic column tube; the center of the sieve plate is perforated; each of the centers of the upper underside and the lower underside of the column core is provided with a small column; and the size of the small column is matched with the aperture of the center of the sieve plate. Through fixing the column core in the chromatographic column tube, the novel liquid phase chromatographic column can basically solve the problem of tail formation commonly existing in chromatographic analysis, thereby improving the resolution of the chromatographic column and having wide application prospect in the fields of chemical engineering, biology, medicine and so on.
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Description

Technical field:

[0001] The invention relates to a novel liquid chromatographic column and a high performance liquid chromatographic analysis method utilizing the chromatographic column. Background technique:

[0002] High-performance liquid chromatography has an irreplaceable position by other separation methods. It has the advantages of high separation efficiency, high selectivity, high detection sensitivity, and fast analysis speed. High performance liquid chromatography can be used not only for analysis and detection, but also for the purification and preparation of samples, especially for the preparation of samples with low content and high added value. However, due to the structural characteristics of the existing chromatographic columns and the operation mode of the chromatographic column, there will always be a problem in the process of using liquid chromatography - chromatographic peak tailing. Lu Peizhang, Zang Chaozheng reported on pages 23, 28, and 93 of "Theory...

Examples

Embodiment Construction

[0019] The inventors deeply studied the reasons for the tailing of traditional chromatographic peaks, and found that: the mobile phase moving between the particles of the stationary phase has different flow velocities for the mobile phase molecules in different laminar flows, and the solute molecules are close to the particles or the edge of the chromatographic column The moving speed of the mobile phase in the laminar flow is slower than that in the central laminar flow, thus causing the peak shape to expand and form a tailing peak.

[0020] For this reason, the inventor boldly imagined improving the structure of the chromatographic column, thereby solving the problem of tailing peaks caused by mass transfer resistance of the mobile phase. The invention fixes a stainless steel column core of a certain size in the chromatographic column tube, so that the mass transfer distance of the solute from the mobile phase laminar flow close to the particle edge to the central laminar flo...