Liquid chromatography column and high performance liquid chromatography analytical method
A liquid chromatographic column and chromatographic column technology, which is applied in the field of new liquid chromatographic column and high performance liquid chromatographic analysis, can solve problems such as chromatographic peak tailing, achieve the effect of improving resolution and broad application prospects
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[0019] The inventors deeply studied the reasons for the tailing of traditional chromatographic peaks, and found that: the mobile phase moving between the particles of the stationary phase has different flow velocities for the mobile phase molecules in different laminar flows, and the solute molecules are close to the particles or the edge of the chromatographic column The moving speed of the mobile phase in the laminar flow is slower than that in the central laminar flow, thus causing the peak shape to expand and form a tailing peak.
[0020] For this reason, the inventor boldly imagined improving the structure of the chromatographic column, thereby solving the problem of tailing peaks caused by mass transfer resistance of the mobile phase. The invention fixes a stainless steel column core of a certain size in the chromatographic column tube, so that the mass transfer distance of the solute from the mobile phase laminar flow close to the particle edge to the central laminar flo...
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