Method for separating and purifying gelsevirine, koumidine, koumine, gelsemine and furan koumine from gelsemium elegans benth

A technology of kelezin and kellycetin, which is applied in the field of natural medicine extraction, can solve the problems of waste of active ingredients, inability to separate multiple drug ingredients at the same time, and low yield of active ingredients, so as to ensure repeated use, low cost, Simple operation effect

Active Publication Date: 2017-09-01
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

[0004] Aiming at the existing technology of separation of the active ingredients of the drug in Gelkinus, it is impossible to separate multiple drug ingredients at the same time, resulting in a waste of active ingredients, and the separated monomers have different processes, and the yield of the active ingredients obtained is low.

Method used

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  • Method for separating and purifying gelsevirine, koumidine, koumine, gelsemine and furan koumine from gelsemium elegans benth
  • Method for separating and purifying gelsevirine, koumidine, koumine, gelsemine and furan koumine from gelsemium elegans benth
  • Method for separating and purifying gelsevirine, koumidine, koumine, gelsemine and furan koumine from gelsemium elegans benth

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Embodiment 1

[0035] Extraction: add 800L of 0.5% sulfuric acid solution to 100kg of crushed Glycyrrhizae, stir and extract in an acid-resistant extraction tank with a capacity of 1000L for 24 hours, filter the medicinal liquid with a 200-mesh filter, extract the medicinal residue once more in the same way, and combine the two The secondary extract is adjusted to neutral pH with sodium hydroxide (8mol / L), then concentrated in a concentration tank to a relative density of about 1.2, and dried under reduced pressure to obtain the product. Weigh 1 kg of gousilic acid water extract, put it into a 5L round-bottomed distillation bottle, add 90% ethanol to reflux and extract twice, add 1.5L each time, extract for 2 hours, and collect the extract twice after simply filtering it through gauze , combined and placed to settle for 2 hours, then centrifuged in a centrifuge to obtain a centrifuge, concentrate the centrifuge to obtain an extract, add hot water at 45-55°C to dissolve, freeze-dry at -20°C fo...

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Abstract

The invention discloses a method for extracting and separating gelsevirine, koumidine, koumine, gelsemine and furan koumine from gelsemium elegans benth. The method comprises the following steps: extracting and neutralizing gelsemium elegans benth raw materials by using an acidic water solution, and drying under reduced pressure, thus obtaining gelsemic acid water extract; extracting the gelsemic acid water extract by using an organic solvent, concentrating, dissolving by water and carrying out freeze drying, thus obtaining gelsemium elegans benth extract powder; separating and purifying the gelsemium elegans benth powder, thus respectively obtaining the gelsevirine, the koumidine, the koumine, the gelsemine and the furan koumine. According to the method disclosed by the invention, the gelsevirine, the koumidine, the koumine, the gelsemine and the furan koumine which are high in purity can be extracted and separated from the gelsemium elegans benth, the operation is simple, the cost is low, simultaneous separation of various alkaloids in the gelsemium elegans benth is realized, and the method is beneficial for comprehensive utilization of natural resources.

Description

technical field [0001] The present invention relates to a method for extracting alkaloids from Gelsemium elegans, in particular to the simultaneous separation and purification of gelsemium chlorophyll, gesemimelegans, gesemimelegans, gesemielegans A and furanose from Gelsemium elegans Benth. The method of kissing Suzi belongs to the field of natural medicine extraction. Background technique [0002] The hook kiss is the whole herb of the Strychniaceae plant, also known as the heartbroken grass, kudzu, poisonous root, big tea medicine, etc. Bitter in the mouth, slightly pungent, hot in nature, very poisonous. Mainly distributed in Zhejiang, Fujian, Guangdong, Guangxi, Hunan, Guizhou and Yunnan. It is commonly used in traditional Chinese medicine to treat rheumatoid arthritis pain, neuropathic pain, and skin ulcers. In terms of veterinary use, a small dose of Gelkins can have a growth-promoting effect on some animals. Gelfish is mainly rich in indole alkaloids. At present...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/18C07D471/22C07D491/22
CPCC07D471/22C07D491/18C07D491/22
Inventor 刘兆颖程辟孙志良
Owner HUNAN AGRICULTURAL UNIV
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