Auto-fluorescence molecule image system

Abstract

The invention belongs to an auto-fluorescence molecule image system which comprises a camera bellows, a CCD camera lens control module, an image processing module, an interactive analysis module and a database module. A system factor corrected by an integrating sphere can be converted into a photon density in an interesting area by the system through a pixel value. The system can work after a temperature value of the CCD camera is set and locked. Firstly, the light source in the camera bellows is opened to shoot an outline of a target object, then the light source is closed to capture a fluorescence signal which projects the outline of the detected object, and the signal is superposed with the outline image. User can choose the interesting area by using a selecting tool so as to acquire a photon density value for performing scientific research. The system of the invention can provide a database function for the user to conveniently manage the data and preview the images. The system of the invention has the advantages of reasonable structure, obvious function, convenient operation, low cost, wide application in the field of researching the small animals in non-invasive biologic experiment.

Description

technical field [0001] The invention belongs to the technical field of molecular imaging, and relates to subject knowledge such as optical imaging theory, computer image processing technology, mathematical modeling and the like. It includes in vivo fluorescence imaging detection equipment, control unit, image processing and data analysis and management, especially a complete set of molecular imaging system suitable for in vivo and non-invasive scientific research on small animals in biology. Background technique [0002] Autofluorescence imaging technology is an emerging optical molecular imaging technology in living animals in recent years. Bioautofluorescence is to mark cells or DNA with luciferase gene, and integrate the plasmid vector containing Fluc gene into the cell or the chromosomal DNA of the cell to express luciferase. In the presence of ATP and oxygen, if the substrate luciferin is injected into a living animal, luciferase will catalyze the oxidation reaction of...

AI Technical Summary

Problems solved by technology

This method cannot adapt to the needs of measured objects of different sizes. When the sizes of the measured objects vary greatly, the fixed four positions may not be able to meet the accurate selection of the visible area.

In terms of data management, it provides folder-style processing, but for long-term and large amounts of experimental data, file search is inconvenient

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