Method for constructing model of mouse of osteoporosis caused by secondary hyperparathyroidism and application of method

A technology of osteoporosis and mouse model, applied in the field of biomedicine, can solve the problems that osteoporosis is easily overlooked, and achieve the effect of high success rate, high clinical relevance, and accurate judgment method

Active Publication Date: 2020-04-14
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, people have gradually realized that the excessive secretion of PTH can also lead to osteoporosis; however, there is still a phenomenon of preference for the main symptom in the current clinical screening, that is, patients with hyperparathyroidism are often focused on the excessive secretion of PTH, which is accompanied by of osteoporosis are easily overlooked
[0004] At present, how to construct an animal model of osteoporosis caused by secondary hyperparathyroidism has not been disclosed in the prior art, which is crucial to the research work of osteoporosis caused by secondary hyperparathyroidism. Therefore, if It is very meaningful to be able to construct an animal model of osteoporosis caused by secondary hyperparathyroidism

Method used

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  • Method for constructing model of mouse of osteoporosis caused by secondary hyperparathyroidism and application of method
  • Method for constructing model of mouse of osteoporosis caused by secondary hyperparathyroidism and application of method
  • Method for constructing model of mouse of osteoporosis caused by secondary hyperparathyroidism and application of method

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Embodiment 1

[0047] This embodiment provides a method for constructing a mouse model of osteoporosis caused by secondary hyperparathyroidism, the construction method comprising:

[0048] (1) Place the parathyroid tissue from a patient with secondary hyperparathyroidism in pre-cooled D-Hanks solution, use fine forceps and surgical scissors to peel off the vascular tissue, and cut it into pieces of 1mm 3 organization block;

[0049] (2) Add culture medium (RPMI1640+10% FBS+1% double antibody) to the 6-well plate, then transfer the tissue block obtained in step (1), and place it in a 37°C, 5% CO2 incubator for 5 days ;

[0050] (3) Use a scalpel to make an opening in the triangular area of ​​the back of the mouse, use tweezers to extend into the opening and widen to form a pocket for holding tissue blocks, then place the four tissue blocks obtained in step (2) into the pockets and suture them, and Disinfect the wound to construct a mouse model of osteoporosis caused by secondary hyperparath...

Embodiment 2

[0052] This embodiment provides a method for constructing a mouse model of osteoporosis caused by secondary hyperparathyroidism, the construction method comprising:

[0053] (1) Place the parathyroid tissue from a patient with secondary hyperparathyroidism in pre-cooled D-Hanks solution, use fine forceps and surgical scissors to peel off the vascular tissue, and cut it into 1.5mm pieces 3 organization block;

[0054] (2) Add culture medium (RPMI1640+10% FBS+1% double antibody) to the 6-well plate, then transfer the tissue block obtained in step (1), and culture it in a 37°C, 5% CO2 incubator for 7 days ;

[0055] (3) Use a scalpel to open an opening in the triangular area of ​​the back of the mouse, use tweezers to extend into the opening and widen to form a pocket for holding the tissue block, then place the three tissue blocks obtained in step (2) into the pocket and suture, and Disinfect the wound to construct a mouse model of osteoporosis caused by secondary hyperparathy...

Embodiment 3

[0057] This embodiment provides a method for constructing a mouse model of osteoporosis caused by secondary hyperparathyroidism, the construction method comprising:

[0058] (1) Place the parathyroid tissue from a patient with secondary hyperparathyroidism in pre-cooled D-Hanks solution, use fine forceps and surgical scissors to peel off the vascular tissue, and cut it into 0.5mm pieces 3 organization block;

[0059] (2) Add culture medium (RPMI1640+10% FBS+1% double antibody) to the 6-well plate, then transfer the tissue block obtained in step (1), and culture it in a 37°C, 5% CO2 incubator for 3 days ;

[0060] (3) Use a scalpel to make an opening in the triangular area of ​​the back of the mouse, use tweezers to extend into the opening and widen to form a pocket for holding the tissue block, then put the five tissue blocks obtained in step (2) into the pocket and suture them, and Disinfect the wound to construct a mouse model of osteoporosis caused by secondary hyperparat...

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Abstract

The invention relates to a method for constructing a model of a mouse of osteoporosis caused by secondary hyperparathyroidism and application of the method. The method comprises the following steps: pretreating parathyroid gland tissue derived from a patient suffering from secondary hyperparathyroidism, transplanting the parathyroid gland tissue into a mouse, and constructing the model of the mouse of osteoporosis caused by secondary hyperparathyroidism. The method for constructing the model of the mouse of osteoporosis caused by secondary hyperparathyroidism, which is provided by the invention, makes a good model construction basis for disease development, treatment medicine screening and scientific evaluation on treatment effects, by adopting the method, pathogenesis severity degrees andtreatment situations of mice can be scientifically, rapidly and accurately judged, and a convenient, rapid and accurate judgment method is also successfully constructed for medicine screening modelsfor treating osteoporosis caused by secondary hyperparathyroidism. The method has a very high success rate and has very high clinical relevance.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for constructing a mouse model of osteoporosis caused by secondary hyperparathyroidism and its application. Background technique [0002] Parathyroid hormone (PTH) is secreted by parathyroid chief cells, an important calcium and phosphorus homeostasis regulator composed of 84 amino acids, which can regulate its own expression and secretion according to changes in blood calcium concentration. It plays an important role in maintaining the body's blood calcium and phosphorus balance and bone metabolism balance. Intermittent administration of low doses of PTH can promote the maturation and differentiation of osteoblasts, mediate the process of osteogenesis, and increase bone mass; while continuous administration of high doses of PTH can promote the maturation of osteoclasts, thereby mediating the process of osteoclasts and inducing osteoclasts. Bone loss. P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61K49/00
CPCA01K67/02A61K49/0008
Inventor 杨帆刘运辉张路
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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