Micro-fluidic chip for cell capture and fluorescent staining

Abstract

The invention provides a micro-fluidic chip for cell capture and fluorescent staining. The micro-fluidic chip comprises a substrate and a cell runner, wherein the cell runner is arranged on the substrate; a sample inlet and a waste liquid outlet are formed in the substrate; one end of the cell runner is a cell inlet; the other end of the cell runner is a cell outlet; the cell inlet is connected with the sample inlet; the cell outlet is connected with the waste liquid outlet; a staining liquid channel is further connected with the cell inlet; a staining liquid inlet is formed in the other end of the staining liquid channel; the cell runner consists of a plurality of micro channels which are arranged in parallel from head to end; a cell capturing device is arranged inside the micro channels. The micro-fluidic chip provided by the invention is high in capturing efficiency and small in cell injury, the integration of enrichment, capturing and staining of target cells can be achieved, artificial interference can be reduced, and the sensitivity and the reliability of results can be improved. The micro-fluidic chip provided by the invention is free of complex process, can be prepared by using a common method, and is low in cost and very good in practicability.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to a microfluidic chip used for cell capture and fluorescent dyeing. Background technique [0002] Microfluidic chip technology integrates the basic operation units such as sample preparation, reaction, separation, and detection in the process of biological, chemical, and medical analysis into a micron-scale chip, and automatically completes the entire analysis process; it is the life science, chemical science and An important technical platform for the research of information science signal detection and processing methods. In recent years, the microfluidic chip has a channel size (10-100um) that is similar to the diameter of a single cell (10-20um), making it easy to operate on cells and respond more quickly and efficiently; the relatively closed environment formed by the microfluidic chip is more The living environment of cells is close to the physiological state; the...

AI Technical Summary

Problems solved by technology

However, solid staining on glass slides generally requires manipulation on the surface of a poly-lysine-coated carrier, which requires certain experimental skills of the operator

And because the slides need to be cleaned repeatedly between each of the above operations, it is easy to cause the loss of cells on the slides. In addition, the cells on the slides are exposed to the air during the processing, which is also easy to cause pollution.

However, liquid staining needs to be detected by flow cytometry, which is costly and requires at least 10 centrifugation operations, which not only consumes a long time and manpower, but also easily causes cell disintegration during the process of discarding the supernatant. loss

[0007] To sum up, the existing cell capture and staining methods have the following problems: after the sample is captured by cells, manual operation is required for cell staining, which is not only cumbersome, but also requires operators to have strong experimental skills. The error is large, which affects the accuracy of the measurement results

And for microfluidic chips to capture target cells, due to the large size of target cells, the flow channel is easy to block, causing target cells to rupture, or cells to escape, resulting in low capture efficiency

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