The invention discloses an artificial freezing stress method for improving
cold resistance of apple trees and promoting growth and development and application of the artificial freezing stress method, and belongs to the technical field of
fruit tree cultivation. The method comprises the following steps: before the
germination stage of the apple trees, when the natural low temperature reaches 0-1 DEG C, manually spraying water to the apple trees in an open-air environment, so that the surfaces of the
fruit tree plants are frozen, and continuous artificial freezing stress is formed; wherein the temperature range of the artificial freezing stress is-2 DEG C to-8 DEG C, and the
duration time is 5-12 hours. The method preferably lasts for 8 to 12 hours, more preferably about 8 hours, and is suitable for red Fuji apple trees. During use, water spraying treatment is performed according to the parameters, and the water spraying amount is based on the condition that tree crowns are uniformly covered and ice
layers are formed. According to the method, the late spring coldness resistance of the apple trees can be effectively improved, the leaf area is promoted to be increased to 2000 mm < 2 > or above, the photosynthetic rate is increased to 8.5 [mu] mol / m < 2 >. S or above, meanwhile, the
proline content of the fruits is increased to 1200 [mu] g / g or above, the soluble
sugar content is increased to 16.0% or above, and CBF and COR cold-resistant
gene expression is activated.