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Method for mutagenizing and screening microalgae with high oil yield by ARTP

A microalgae and high-yield technology, applied in the biological field, can solve the problems of low oil content, inability to fully utilize the energy advantages of microalgae biomass, and high oil content

Inactive Publication Date: 2020-03-17
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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Problems solved by technology

However, the biomass and oil content of different algae species are different, and the biomass yield and oil content are not positively correlated. Low trend; in addition, under environmental stress, Chlorella biomass showed lower levels, but oil content was higher
Therefore, a single selection of mutant algal strains with high biomass or high oil content cannot fully utilize the advantages of microalgal biomass energy

Method used

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  • Method for mutagenizing and screening microalgae with high oil yield by ARTP
  • Method for mutagenizing and screening microalgae with high oil yield by ARTP
  • Method for mutagenizing and screening microalgae with high oil yield by ARTP

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Embodiment

[0057] This embodiment takes Scenedesmus as an example to describe the method in detail. Scenedesmus was purchased from the Freshwater Algae Species Bank of Wuhan Institute of Hydrobiology, Chinese Academy of Sciences. The ARTP mutagenesis system used was purchased from Nanjing Suman Plasma Technology Co., Ltd.

[0058] 1. Use BG11 medium to cultivate algae, inoculate 200mL Scenedesmus liquid into a 500mL triangular conical flask, seal the bottle mouth with a sterile film, place it in a constant temperature light incubator, and cultivate it in a semi-continuous culture mode. Average light intensity: 80μmol m -1 ·s -1 , photoperiod: 12L:12D, cultivation temperature: (25±1)℃. Change the position of the Erlenmeyer flask randomly every day and shake it 3-4 times.

[0059] 2. To be cultured to the logarithmic phase, the cell concentration is 10 6 At this time, the cell viability is strong and it is more suitable for mutagenesis. Take 0.1 mL of algae liquid and put it in a steri...

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Abstract

The invention discloses a method for mutagenizing and screening microalgae with a high oil yield by ARTP. The method mainly comprises the following two processes: (1) mutagenizing microalgae by usingARTP, and determining the optimal mutagenizing time by adjusting the influence of mutagenizing time on the mutagenizing effect and taking a fatality rate as the basis, and (2) carrying out gradient dilution (capable of efficiently and accurately counting) on the mutated algae liquid at each mutagenesis time, uniformly coating the mutated algae liquid on a solid culture medium, carrying out inverseculture in an illumination incubator for 10 days, selecting the algae colonies with the dark color and larger diameter, inoculating a liquid culture medium with the algae colonies, measuring the absorbance of the algae liquid and the fluorescence value of neutral grease dyed by Nile red to know the cell proliferation rate and grease accumulation condition of microalgae, through comprehensive screening of two basic indexes and the product of the two basic indexes, screening a mutagenic algae strain with a high grease yield, so that the possibility of preparing biodiesel from microalgae is further improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for mutagenizing and screening microalgae with high oil-yielding rate by using ARTP. Background technique [0002] Due to the non-renewability of fossil fuels and the impact of their combustion on the environment, clean fuels and renewable new energy have attracted widespread attention from countries all over the world today. Biomass energy is the most common renewable energy on the earth. It is a form of energy that stores solar energy in the form of chemical energy in organisms through plant photosynthesis, and is called green energy. Biodiesel refers to the fatty acid methyl or ethyl ester formed by the transesterification of oil from living organisms. It is a kind of biomass liquid fuel and has become a hot spot in the research of biomass energy in recent years. [0003] At present, the key problem restricting the development of biodiesel is the serious shor...

Claims

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Application Information

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IPC IPC(8): C12N15/01C12N13/00C12R1/89
CPCC12N13/00C12N15/01
Inventor 孙昕李鹏飞黄峰王瑾
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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