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Method for improving genetic characteristics in diatom oil production

A technology of genetic characteristics and diatoms, which is applied in the field of improving genetic characteristics of diatom oil production and mutant algae strains, can solve problems such as increased oil production, reduced proportion of unsaturated fatty acids, increased oil production of microalgae, growth inhibition, etc., to achieve The effect of increased oil accumulation

Inactive Publication Date: 2012-12-19
INST OF AQUATIC LIFE ACAD SINICA +1
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Problems solved by technology

[0011] The purpose of the present invention is to overcome the problem in the prior art that it is difficult to increase the oil production of microalgae or cause growth inhibition by using gene silencing or expressing a single gene in the oil production pathway, and to provide a method for improving the genetic characteristics of diatom oil production. method; this method is the first time in a diploid microalgae to use exogenous genes to randomly integrate into the genome to establish a mutant library, and through screening to obtain mutants with increased oil production, reduced proportion of unsaturated fatty acids, and unaffected growth algal strain

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  • Method for improving genetic characteristics in diatom oil production
  • Method for improving genetic characteristics in diatom oil production
  • Method for improving genetic characteristics in diatom oil production

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example 1

[0058] The mutant strain 4-2411 selected in Example 1 is a high-yielding oil strain, and its triglyceride content is not only 4.69% higher than that of the wild type, but the proportion of polyunsaturated fatty acids in the fatty acid composition is also significantly lower than that of the wild type, reaching the European Union's established Standard, except for the cost savings of not requiring saturation modification. According to a conservative estimate, for every 1% increase in the triglyceride content of oleaginous algae, an additional 400L of biodiesel will be produced per hectare per year. The current petroleum diesel price is 7.5 yuan / L as the standard, and the mutant strain 4-2411 To produce biodiesel as raw material, the output value per unit area should be saved at least 14,070 yuan per year.

[0059] 2. Using Phaeodactylum tricornutum ( P. tricornutum ) to construct a library of random insertion mutants, and analyze the fatty acid composition of mutant strains b...

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Abstract

The invention discloses a method for improving genetic characteristics in diatom oil production, and relates to the genetic breeding method of diatom. The method comprises the following steps: (1) establishing karyogene transformation plasmid; (2) transferring the transformation plasmid into the genome of the diatom to establish a random inserting mutant library; (3) sorting high oil-producing mutant strains in the mutant library through a Nile red fluorescence staining ratio method; (4) measuring triglyceride in the cell, analyzing the fatty acid; and (5) measuring the growth. With adoption of the method, fine varieties can be selected and cultivated by randomly inserting mutation; and the method can be applied to any diatom in which a genetic material transfer system is successfully established; the strains with good comprehensive performance can be successfully sieved easier than gene expression or silence; compared with the methods of chemical mutagenesis and irradiation mutagenesis, the method has no harm to the human body. According to the method, the oil accumulation of the sorted high oil-producing mutant strains is increased, and moreover, the ratio of polyunsaturated fatty acids in the triglyceride can be reduced, so that the triglyceride can be served as the raw material for producing biodiesel without being subject to saturation modification.

Description

Technical field [0001] The present invention involves a type of oil -producing autonomy -the genetic breeding method of diatoms, especially a method for improving the genetic characteristics of diatomic oil production; specifically, the present invention uses genetic operations, and the invention uses genetic operations, and the invention uses genetic operations, and the invention uses genetic operations, and the invention uses genetic operations, and the invention uses genetic operations, and the invention uses genetic operations, and the invention uses genetic operations, and the invention uses genetic operations, and the invention uses genetic operations, and the present invention uses genetic operations.Inserted the external genes into the nuclear group, build a mutant library, and screen a mutant strain with high -yield oil and low unsaturated fatty acids. Background technique [0002] The large -scale continuous consumption of fossil fuels on the earth will cause global ene...

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Application Information

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IPC IPC(8): C12N15/63C12N1/13C12R1/89
Inventor 孔任秋徐旭东刘飞飞方仙桃
Owner INST OF AQUATIC LIFE ACAD SINICA
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