Method of creating zebra fish peristalsis model and screening prokinetic medicaments

Abstract

The invention belongs to the field of drug screening and particularly relates to a method of creating a zebra fish peristalsis model and screening prokinetic medicaments. The method comprises the following steps of: (1) selecting the zebra fish; (2) inducing the model and treating the compound; (3) quantitatively analyzing through a fluorescence microscope. A fluorescent dye is applied in the invention for the first time to research the gastrointestinal peristalsis conditions, the new research method is a great complement for the existing research models, and the peristalsis model has important value in high throughput screening of the prokinetic compounds due to the maneuverability, non-invasion, simplicity and quickness.

Description

technical field [0001] The invention belongs to the field of drug screening (evaluation), and specifically relates to a simple, economical, fast, efficient, and high-throughput method for establishing a zebrafish intestinal peristalsis model, and uses the animal model to screen drugs for promoting gastrointestinal motility and evaluate drug effects on Stimulation of bowel movements. Background technique [0002] Gastrointestinal motility disorders are common clinical diseases. In daily medical work, about 50% of patients seek medical treatment due to gastrointestinal symptoms, and 30%-40% of them are finally diagnosed as gastrointestinal motility disorders, such as gastroesophageal reflux disease (GERD), Functional dyspepsia (FD), gastroparesis, intestinal pseudo-obstruction, irritable bowel syndrome and chronic constipation [[i]] . Gastrointestinal motility disorders include weak gastrointestinal contractions, rhythm disturbances, and abnormally directional contractions. ...

AI Technical Summary

Problems solved by technology

The third generation is cisapride, which is a kind of mehydroclopramide that replaces the alkylated phenylpropamide in its chemical structure. In recent years, it has been clinically found that Q-T interval prolongation or torsades de pointes can occur in patients with high sensitivity to cisapride. Ventricular tachycardia [[v]], there is still a lot of controversy about its further clinical application

Due to its complexity, gastrointestinal motility models face many challenges

In vitro cell experiments lack tissue and organ structures and cannot accurately simulate gastrointestinal motility

Rats, pigs and other isolated organs lack peripheral nerve feedback and cannot accurately simulate gastrointestinal motility[[x],[xi]]

Mammalian experiments use imaging technology to observe gastrointestinal motility, which is time-consuming, laborious and expensive[[xii],[xiii]]

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