Method of creating zebra fish peristalsis model and screening prokinetic medicaments

A gastrointestinal motility and zebrafish technology, applied in the field of establishment of zebrafish intestinal peristalsis model, can solve the problems of inaccurate simulation of gastrointestinal motility, lack of peripheral nerve feedback, prolongation of Q-T interval, etc.

Active Publication Date: 2013-09-18
HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The third generation is cisapride, which is a kind of mehydroclopramide that replaces the alkylated phenylpropamide in its chemical structure. In recent years, it has been clinically found that Q-T interval prolongation or torsades de pointes can occur in patients with high sensitivity to cisapride. Ventricular tachycardia [[v]], there is still a lot of controversy about its further clinical application
Due to its complexity, gastroin

Method used

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  • Method of creating zebra fish peristalsis model and screening prokinetic medicaments
  • Method of creating zebra fish peristalsis model and screening prokinetic medicaments
  • Method of creating zebra fish peristalsis model and screening prokinetic medicaments

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0130] Example 1 Method for Establishing a Zebrafish Intestinal Motility Model

[0131] Based on the optimal treatment stage of zebrafish, the optimal treatment time length and concentration of the nile red compound, and the optimal treatment time length of the compound, the design scheme is as follows:

[0132] 1 Zebrafish Selection

[0133] The 5 dpf zebrafish were observed under a dissecting microscope, and the normally developed zebrafish were picked and transferred into 6-well plates, 30 in each well.

[0134] deal with

[0135] Set up 5 experimental groups: remove the culture water in the microwell plate, add 3mL 10ng / mL Nile red staining solution to the 5 experimental groups, put the microwell plate into a constant temperature incubator at 28°C for 16h respectively.

[0136] Compound treatment

[0137] Set up 5 experimental groups: 3 concentration morphine groups, 1 solvent control group, and 1 model group. Remove the Nile red staining solution in the microw...

Embodiment 2

[0143] Example 2 Method for Screening Drugs Promoting Gastrointestinal Motility Using Live Zebrafish

[0144] 1 Zebrafish Selection

[0145] The 5 dpf zebrafish were observed under a dissecting microscope, and the normally developed zebrafish were picked and transferred into 6-well plates, 30 in each well.

[0146] deal with

[0147] Set up 6 experimental groups: Remove the culture water in the microwell plate, add 3mL 10ng / mL Nile red staining solution to the 6 experimental groups, put the microwell plate into a constant temperature incubator at 28°C for 16h respectively.

[0148] Compound treatment

[0149] Set up 6 experimental groups: 3 concentrations of test compound treatment groups, 1 positive control group, 1 solvent control group, and 1 model group. Remove the Nile red dye solution in the microwell plate and wash it quickly with aquaculture water for 3 The zebrafish were transferred to a new microwell plate, and the three compounds were treated with magnesi...

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Abstract

The invention belongs to the field of drug screening and particularly relates to a method of creating a zebra fish peristalsis model and screening prokinetic medicaments. The method comprises the following steps of: (1) selecting the zebra fish; (2) inducing the model and treating the compound; (3) quantitatively analyzing through a fluorescence microscope. A fluorescent dye is applied in the invention for the first time to research the gastrointestinal peristalsis conditions, the new research method is a great complement for the existing research models, and the peristalsis model has important value in high throughput screening of the prokinetic compounds due to the maneuverability, non-invasion, simplicity and quickness.

Description

technical field [0001] The invention belongs to the field of drug screening (evaluation), and specifically relates to a simple, economical, fast, efficient, and high-throughput method for establishing a zebrafish intestinal peristalsis model, and uses the animal model to screen drugs for promoting gastrointestinal motility and evaluate drug effects on Stimulation of bowel movements. Background technique [0002] Gastrointestinal motility disorders are common clinical diseases. In daily medical work, about 50% of patients seek medical treatment due to gastrointestinal symptoms, and 30%-40% of them are finally diagnosed as gastrointestinal motility disorders, such as gastroesophageal reflux disease (GERD), Functional dyspepsia (FD), gastroparesis, intestinal pseudo-obstruction, irritable bowel syndrome and chronic constipation [[i]] . Gastrointestinal motility disorders include weak gastrointestinal contractions, rhythm disturbances, and abnormally directional contractions. ...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 周娟郭胜亚李春启
Owner HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
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