Compound fluorescent micro-nano system and preparation method thereof based on one-pot process

A technology of composite fluorescence and preparation process, which is applied in the field of composite fluorescence micro-nano system and its preparation process based on one-pot cooking method, which can solve the problems of complex steps, toxicity, and inability to evaluate the biological safety of polymers in vivo, and achieve the goal of targeting and release, expand the preparation process, and improve the effect of bioavailability

Inactive Publication Date: 2015-09-02
WENZHOU INST OF BIOMATERIALS & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the micro-nano systems reported in the literature in the past use phospholipids, albumin, sugars, etc. as matrix materials, and the construction strategies are mainly layer-by-layer superposition, water-oil-water, oil-water-oil microemulsion and other methods, and there are complicated steps. , poor system stability, etc.
Although the use of polymers as matrix materials can improve their stability, the synthesis process is complex and the yield is low, and toxic and polluted organic solvents are often used as media, and the in vivo biosafety of the obtained polymers cannot be evaluated.

Method used

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  • Compound fluorescent micro-nano system and preparation method thereof based on one-pot process
  • Compound fluorescent micro-nano system and preparation method thereof based on one-pot process
  • Compound fluorescent micro-nano system and preparation method thereof based on one-pot process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Butyl acrylate (1.5 ml), polyethylene glycol octyl phenyl ether aqueous solution (250 ml), and rhodamine (9.6 mg) were fed into a 500 ml beaker, and the pH of the reaction system was adjusted to 2.5. After stirring at a high speed at room temperature for 1 hour, dilute sodium hydroxide solution was added to terminate the reaction. The reaction system was transferred to a separatory funnel and left to stand for 6 hours, and the reaction residue at the bottom layer was discarded. Transfer the reaction solution to a centrifuge tube for purification and centrifugation for 20 minutes, carefully collect the top product layer, and wash with polyethylene glycol octylphenyl ether solution. Repeat the above steps at least 2-4 times, re-disperse into the polyethylene glycol octyl phenyl ether solution after concentration, and store at room temperature. The particle size of the composite fluorescent micro-nano system containing rhodamine was measured by laser particle s...

Embodiment 2

[0030] Example 2: Butyl acrylate (1.5 ml), dextran aqueous solution (100 ml), and rhodamine (9.6 mg) were fed into a 250 ml beaker, and the pH of the reaction system was adjusted to 2.5. After magnetic stirring at room temperature for 4 hours, dilute sodium hydroxide solution was added to terminate the reaction. The reaction system was left to stand for 6 hours, then all the reaction solution was transferred to a centrifuge tube and centrifuged for 30 minutes, the supernatant was discarded, washed with high-purity water, and vortexed several times. The centrifugation step is repeated at least 2-4 times, and after concentrating, a composite fluorescent micro-nano system containing rhodamine is obtained, which can be freeze-dried in vacuum or dispersed in high-purity water for storage again. As measured by a laser particle size analyzer, the particle size of the rhodamine-containing composite fluorescent micro-nano system is 252.8±46.2 nanometers, and the surface potential is -3...

Embodiment 3

[0031] Example 3: Butyl acrylate (1.5 ml), polyethylene glycol octyl phenyl ether aqueous solution (250 ml), and coumarin 6 (7.0 mg) were fed into a 500 ml beaker, and the pH of the reaction system was adjusted to 2.5. After stirring at a high speed at room temperature for 1 hour, dilute sodium hydroxide solution was added to terminate the reaction. The reaction system was transferred to a separatory funnel and left to stand for 6 hours, and the reaction residue at the bottom layer was discarded. Transfer the reaction solution to a centrifuge tube for purification and centrifugation for 20 minutes, carefully collect the top product layer, and wash with polyethylene glycol octylphenyl ether solution. Repeat the above steps at least 2-4 times, re-disperse into the polyethylene glycol octyl phenyl ether solution after concentration, and store at room temperature. The particle size of the composite fluorescent micro-nano system loaded with coumarin 6 is 4.17±0.96 microns and the su...

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Abstract

The invention discloses a compound fluorescent micro-nano system and a preparation method thereof based on a one-pot process. A biomedical material with the matrix being butyl polyacrylate is constructed, wherein the entrapment component can be selected from fluorescent substances such as rhodamine, coumarin 6, nile red, camptothecin, adriamycin amycin and the like. The system disclosed by the invention is simple in preparation process, mild in condition, green and environment-friendly in process, less in energy consumption, free of pollution of three wastes, radiation, noise and the like and simple and convenient in separation and purification process. The obtained system is stable and easy to store and is a universal process for selectively preparing the compound fluorescent micro-nano system. Based on good biocompatibility and stability of the compound fluorescent micro-nano system as well as the fluorescence imaging and treating functions of the entrapment component, the system is expected to be relatively widely applied in the field of in vivo marking and tracing, biomedical imaging, targeted diagnosis and treat integration, medicine screening and optimization and the like and has good economic and social benefits in the field of life health and personal medical treatment and the like.

Description

technical field [0001] The invention relates to a fluorescent micro-nano material, in particular to a composite fluorescent micro-nano system and its preparation process based on a one-pot cooking method. Background technique [0002] Fluorescence imaging, as a common imaging technology, has been widely used in biological detection and identification, medical imaging and other fields due to its intuitive and in-situ visual detection means. Fluorescence imaging technology based on fluorescence signal reconstruction has many unique advantages, such as strong sample penetration, high sensitivity and selectivity, showing a good application prospect. Fluorescent imaging probes are widely used in the fields of chemistry, biology, and medicine, but small molecules of traditional organic dyes have some insurmountable defects, such as: unstable properties, easy to be photobleached, and cannot be used for a long time; organic dyes are not suitable for multicolor imaging , can only be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C09K11/02B82Y30/00B82Y40/00
Inventor 刘哲宋远会李宜鸿石长灿汪婕
Owner WENZHOU INST OF BIOMATERIALS & ENG
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