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Ratio fluorescent nano hydrogel for pH value sensing and preparation method thereof

A nano-hydrogel and ratiometric fluorescence technology, which is applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, and luminescent materials, can solve the problems of weakened detection sensitivity and achieve stable and reliable sensing signals

Inactive Publication Date: 2010-06-30
BEIJING JIAOTONG UNIV
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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the defect that the detection sensitivity of a single fluorescence intensity will be weakened due to the distribution and concentration of the probe and the random drift of the optoelectronic system, and provide a ratiometric fluorescence sensor for pH value sensing. Nano hydrogel and preparation method thereof

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  • Ratio fluorescent nano hydrogel for pH value sensing and preparation method thereof
  • Ratio fluorescent nano hydrogel for pH value sensing and preparation method thereof
  • Ratio fluorescent nano hydrogel for pH value sensing and preparation method thereof

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Embodiment Construction

[0028] The present invention will be further described with reference to the drawings and specific embodiments below.

[0029] The ratio fluorescent nano-hydrogel used for pH value sensing proposed in the present invention is a multi-doped nano-hydrogel, wherein the mass percentage of each component is polyurethane: bromothymol blue: incense Soybean 6: Nile Red is 1:0.05:0.05:0.005.

[0030] The preparation method of the ratio fluorescent nano hydrogel for pH value sensing, the steps are as follows:

[0031] Step 1, weighing 10 mg of polyurethane, 0.5 mg of bromothymol blue, 0.5 mg of coumarin 6 and 0.05 mg of Nile red according to the mass percentage of 1:0.05:0.05:0.005;

[0032] Step 2: Mix 22.23ml of ethanol and 2.47ml of water to obtain a 9 / 1 volume ratio ethanol / water mixed solution, add the 10mg of polyurethane weighed in step 1, and add the above-mentioned ethanol / water to mix with magnetic stirring In the solution, it is prepared into a solution with a polyurethane ...

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Abstract

The invention relates to ratio fluorescent nano hydrogel for pH value sensing and a preparation method thereof, belonging to the field of pH value sensing materials. The method comprises the following steps of: weighing polyurethane, bromothymol blue, coumarin 6 and Nile red according to a mass percent of 1:0.05:0.05:0.005; adding the weighed polyurethane to a mixed solution of alcohol and water with a volume ratio that the alcohol to the water is 9:1 to prepare a solution with a concentration of 500ppm; dissolving the bromothymol blue, the coumarin 6 and the Nile red together in the obtained solution and stirring for 1h; dialyzing for 24h in secondary distilled water and changing water every 4h to obtain a hydrogel suspension; and filtering the prepared suspension to obtain a product. The invention overcomes the defect that the detection sensitivity with single fluorescence intensity can be weakened because of the distribution and the concentration of probes and the random drift of an optoelectronic system and is suitable to be used as a pH value detection material in cells.

Description

technical field [0001] The invention relates to a ratio fluorescent nano hydrogel for pH value sensing and a preparation method thereof, belonging to the technical field of pH value sensing materials. Background technique [0002] pH value detection plays a very important role in various fields such as biology and medicine. Among the various materials used for pH detection, hydrogels have attracted much attention due to their high stability, biocompatibility, and stress reactivity (volume change with external stimuli). A variety of nanohydrogels have been developed and used to detect various analytes. However, since the detection mechanism of current nano-hydrogels is mostly based on volume change, it must be integrated or attached to a micro-device to adapt to optical or electrical measurement, and the result can only be detected in the macroscopic field. Recent studies have reported that fluorescent nanohydrogels were prepared by changing the relationship between the vol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/80C09K11/06
Inventor 彭洪尚谢蒂旎黄世华
Owner BEIJING JIAOTONG UNIV
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