Establishment method for alcoholic fatty liver zebra fish model
A technology of hepatic zebrafish and a construction method, which is applied in the field of model animal disease model construction, can solve the problems that embryo model cannot be used as a drug screening scheme, the similarity of disease models is poor, and the law of occurrence and development of alcoholic fatty liver cannot be effectively understood, etc. To achieve the effect of reducing the cost of pharmacological experiments and drug screening, good similarity, and short cycle
Inactive Publication Date: 2016-03-16
NANJING UNIV OF SCI & TECH
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However, at present, although some people use zebrafish embryos to construct alcoholic fatty liver models, the disadvantages are obvious: using zebrafish embryos as experimental animals, the disease model replicated is poorly similar to human diseases, and it cannot accurately induce diseases with similar diseases. The occu
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Embodiment 1
[0054] The present embodiment provides a method for constructing a zebrafish model of alcoholic fatty liver disease, comprising the following steps:
[0055] Step 1) The purchased zebrafish are wild-type zebrafish of the ray-finned fish class, Cyprinidae, Cyprinidae, and AB lineage, with a body length of 2.8-3.2 cm and a weight of 0.20-0.25 g; the conditions for domestication in the laboratory The temperature of the feeding water is controlled at 28.5°C in a 30×25×25cm polycarbonate tank, and the water quality conditions are as follows: conductivity (200μS / cm), hardness (116mg / LCaCO 3 ), dissolved oxygen (O 2 ≥80%, saturation 7.75±0.23mg / LO 2 ), PH value (7.5±0.5), nitrite (+ and NO 2- ), nitrate (≤1.9mg / LNO 3- ), chlorine and free chlorine (2 ), cyanide (0.002mg / LCN - ), total dissolved solids (<168mg / L), total particulate matter (<20mg / L). The water used for fish farming has been fully oxygenated, aerated and dechlorinated for 24 hours. The water is changed once a day a...
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Abstract
The invention discloses an establishment method for an alcoholic fatty liver zebra fish model. The establishment method includes the following steps that AB-series wild zebra fishes are domesticated in a laboratory, and the healthy adult zebra fishes are picked out to be evenly divided into 16 groups at random; a central combinational design method is adopted, and the ethyl alcohol concentration, the exposure time and the test cycle are designed; after the adult zebra fishes in all the groups are fed according to design conditions, the survival rates and average lipid droplet areas of the adult zebra fishes in the groups are calculated; the obtained 16 groups of survival rates and average lipid droplet areas are subjected to standard deviation standardization, survival rate standardized values and average lipid droplet area standardized values of all the groups are added and then divided by 2 to obtain the comprehensive grades of the adult zebra fishes in the groups; the comprehensive grades of all the groups are subjected to multivariate regression analysis and binomial fitting to obtain a regression equation, and the optimal modeling condition of the alcoholic fatty liver zebra fish model is determined according to the regression equation. The establishment method is low in cost and short in cycle, the alcoholic fatty liver pathomechanism can be deeply researched, and a novel treatment means is found for the alcoholic fatty liver.
Description
technical field [0001] The invention relates to the technical field of construction methods for model animal disease models, in particular to a construction method for a zebrafish model of alcoholic fatty liver disease. Background technique [0002] Alcoholic fatty liver is a pathological process of liver damage and excessive accumulation of lipids in liver cells caused by long-term heavy drinking. It is a type of alcoholic liver disease. The morphological changes and metabolic changes of hepatocytes in the process of alcoholic steatosis will bring more and more serious damage to the hepatocytes and affect the normal function of the liver. Compared with other types of fatty liver, alcoholic fatty liver is more likely to induce liver fibrosis and cirrhosis. 20% to 30% of fatty liver caused by ethanol may develop into cirrhosis and even lead to liver cancer. Although there are many theories and hypotheses about the pathogenesis of alcoholic fatty liver, none of them can cove...
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Login to view more IPC IPC(8): C12N5/071C12Q1/02
Inventor 汪俊松陈婷李明会
Owner NANJING UNIV OF SCI & TECH
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