Amphiphilic lipid-droplet fluorescent probe with super-high selectivity and application thereof

A fluorescent probe and selective technology, applied in the lipid droplet fluorescent probe and its application field, can solve the problems of difficulty in imaging lipid droplets with high selectivity, hindering further research on lipid droplets, and failing to meet research needs, and achieve rapid Dyeing ability, good light stability, fast dyeing effect
CN106905309AActive Publication Date: 2017-06-30SHANDONG UNIV
2 Cites 20 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANDONG UNIV
Publication Date
2017-06-30

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an amphiphilic lipid-droplet fluorescent probe with super-high selectivity. The amphiphilic lipid-droplet fluorescent probe with super-high selectivity is characterized in that chemical name of the probe is 1-(3'-(7'-nitrobenzofurazan-4'-)aminopropyl)-2,3,3-trimethylindole bromine, NII for short; and the general chemical formula is as shown in the formula (I). The invention also discloses application of the probe in specifically marking or displaying the form and distribution of lipid droplets in living cells or tissues. Experiments confirm that the probe of the invention is a brand-new probe, has a wide range of application, has good single- and two-photon photostability, fast staining speed and low cytotoxicity, can specifically image lipid droplets in living cells and has a wide application prospect.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to a lipid droplet fluorescent probe and its application, in particular to a super-selective amphiphilic lipid droplet probe and its ability to specifically label or display lipid droplets in living cells or tissues Applications in Morphology and Distribution. Background technique

[0002] Lipid droplets have long been recognized as simple, inert spheres that store neutral lipids (triglycerides and cholesteryl esters) in cells. However, recent studies have shown that lipid droplets are not static, but highly dynamic organelles in cells, widely present in eukaryotic and prokaryotic cells. They play important roles in cells and participate in various activities in cells, such as regulating the metabolism of intracellular lipids, maintaining intracellular balance, and interacting with various organelles in cells. Moreover, the abnormality of lipid droplets can lead to many diseases, such as obesity, diabetes, atherosclerosi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More