Fluorescent probe and application thereof

Abstract

The present invention provides a fluorescent probe and application thereof. The probe which is of an electronic structure of a donor-Pi-receptor has twisted intramolecular charge transfer and aggregation-induced luminescence, and can be excited by two-photon. The probe can help overcome a self-absorption problem caused by large commercial lipid droplet fluorescent dye background and small Stokes'displacement and is high in biocompatibility, high in brightness, low in background and good in light stability. At the same time, a two-photon excitation process is used in the probe, autofluorescence can be lowered, a signal-to-noise ratio can be improved, and a three-dimensional resolution and light stability can be improved; the probe proposed in the invention can be applied to lipid droplet imaging of various cells and tissue sections.

Description

technical field [0001] The present invention relates to two-photon fluorescent probes with aggregation-induced luminescent characteristics and their application in lipid droplet imaging in biological cells. Background technique [0002] Lipid droplets (Lipid droplets, LDs) are composed of phospholipid single-molecule surface layer and neutral lipid inner core, which are the main storage places of neutral lipids in cells, and widely exist in a variety of animal and plant cells. Recent studies have found that lipid droplets are not an "inert" energy storage, but an active multifunctional organelle, and its abnormalities are associated with diseases such as obesity, type II diabetes, fatty liver, hyperlipidemia and atherosclerosis are closely related. Therefore, the detection of lipid droplets is of great significance to biomedical research and clinical diagnosis. In recent years, fluorescence detection method has gradually become an important research method in biomedical re...

AI Technical Summary

Problems solved by technology

Commercial lipid droplet fluorescent dyes mainly include NileRed (NileRed) and BODIPY493 / 503, but they still have some important defects: strong fluorescent background and small Stokes shift

[0003] However, these AIE lipid droplet fluorescent probes still have the problem of short excitation wavelength, resulting in strong background fluorescence and low penetration depth in tissue sections.

To solve these problems, a lot of effort has been devoted to the synthesis of fluorescent dyes excited by long wavelengths, but there are few successful examples

The main challenge of this scheme is that increasing the intermolecular conjugation causes synthesis difficulties, the molecular weight becomes larger, the molecular hydrophobicity increases, the cell penetration decreases, and the quantum yield of red light is low.

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