Carbon quantum dot fluorescent probe for lipid droplet specific labeling as well as preparation method and application thereof

A technology of carbon quantum dots and fluorescent probes, applied in the field of fluorescent biosensing, can solve the problems of poor stability and high cost, and achieve the effects of increased fluorescence intensity, high signal-to-noise ratio, and low cytotoxicity

Active Publication Date: 2021-02-12
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the problems of poor stability and high cost of fluorescent probes currently used for lipid droplet imaging, the purpose of the present invention is to provide a carbon quantum dot fluorescent probe for specific labeling of lipid droplets and a preparation method thereof. Carbon quantum dot fluorescent probe can realize specific imaging of lipid droplets, and has excellent photostability, high quantum yield, high signal-to-noise ratio, etc.

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  • Carbon quantum dot fluorescent probe for lipid droplet specific labeling as well as preparation method and application thereof
  • Carbon quantum dot fluorescent probe for lipid droplet specific labeling as well as preparation method and application thereof
  • Carbon quantum dot fluorescent probe for lipid droplet specific labeling as well as preparation method and application thereof

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Embodiment 1

[0046] The preparation method of the carbon quantum dot fluorescent probe provided in this embodiment, such as figure 1 shown, including the following steps:

[0047] (1) To prepare the precursor reaction solution, put 2g of o-phenylenediamine and 0.5g of thiourea into a beaker, then add 30mL of N,N-dimethylformamide (DMF) and 10mL of deionized water into it, and stir it magnetically Completely dissolve to obtain the precursor reaction solution;

[0048] (2) To prepare carbon quantum dots, place the precursor reaction solution in a 100mL reactor, and then raise the temperature to 210°C for 10 hours; after the reaction is completed, allow the reactor to cool naturally to room temperature;

[0049] (3) to obtain carbon quantum dots, the resulting reaction solution is first filtered with a 0.22 μm filter membrane to remove large particle reaction products; then, the filtered reaction solution is separated through a silica gel chromatographic column (dichloromethane and methanol ...

Embodiment 2

[0051] The preparation method of the carbon quantum dot fluorescent probe provided in this embodiment, such as figure 1 shown, including the following steps:

[0052] (1) To prepare the precursor reaction solution, put 2g of o-phenylenediamine and 0.5g of thiourea into a beaker, then add 30mL of N,N-dimethylformamide (DMF) and 10mL of deionized water into it, and stir it magnetically Completely dissolve to obtain the precursor reaction solution;

[0053] (2) To prepare carbon quantum dots, place the precursor reaction solution in a 100mL reactor, then raise the temperature to 170°C and react for 12 hours; after the reaction is completed, allow the reactor to cool down to room temperature naturally;

[0054] (3) to obtain carbon quantum dots, the resulting reaction solution is first filtered with a 0.22 μm filter membrane to remove large particle reaction products; then, the filtered reaction solution is separated through a silica gel chromatographic column (dichloromethane an...

Embodiment 3

[0056] The preparation method of the carbon quantum dot fluorescent probe provided in this embodiment, such as figure 1 shown, including the following steps:

[0057] (1) To prepare the precursor reaction solution, put 2g of o-phenylenediamine and 0.5g of thiourea into a beaker, then add 30mL of N,N-dimethylformamide (DMF) and 10mL of deionized water into it, and stir it magnetically Completely dissolve to obtain the precursor reaction solution;

[0058] (2) To prepare carbon quantum dots, place the precursor reaction solution in a 100mL reactor, and then raise the temperature to 240°C for 8 hours; after the reaction is completed, allow the reactor to cool down to room temperature naturally;

[0059] (3) to obtain carbon quantum dots, the resulting reaction solution is first filtered with a 0.22 μm filter membrane to remove large particle reaction products; then, the filtered reaction solution is separated through a silica gel chromatographic column (dichloromethane and metha...

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Abstract

The invention discloses a carbon quantum dot fluorescent probe for lipid droplet specific labeling as well as a preparation method and application of the carbon quantum dot fluorescent probe, the carbon quantum dot fluorescent probe takes o-phenylenediamine and thiourea as raw materials, a product containing bright yellow orange fluorescent components is obtained through solvothermal reaction at 170-240 DEG C, and finally the carbon quantum dot fluorescent probe is obtained through filtration, separation and drying. The prepared carbon quantum fluorescent probe has good solubility and bright fluorescence performance in a non-polar hydrophobic oily medium, compared with weak fluorescence performance shown in an aqueous solution, the fluorescence intensity is improved by about 140 times, andthe carbon quantum fluorescent probe can be used for lipid droplet specific fluorescence imaging and has a high signal-to-noise ratio. The carbon quantum dot fluorescent probe provided by the invention can realize specific targeting of intracellular lipid droplets, is novel in performance, and has the characteristics of high fluorescent quantum yield, good light stability, low cytotoxicity, goodbiocompatibility and the like, so that the carbon quantum dot fluorescent probe has a wide application prospect in lipid droplet specific biological imaging.

Description

technical field [0001] The invention belongs to the technical field of fluorescent biosensing and relates to a carbon quantum dot fluorescent technology, in particular to a carbon quantum dot fluorescent probe for specific labeling of lipid droplets and its application. Background technique [0002] Lipid droplets (LDs), also known as fat bodies or liposomes, are intracellular lipid-rich organelles that regulate the storage of neutral lipids, including triglycerides and cholesteryl esters. Although LDs are predominantly found in adipose tissue, almost all cells are able to store lipids in these depots because the ability to store this source of metabolic energy is essential for survival. It has been shown that cells other than adipocytes can form lipid droplets in response to stress. Lipid droplets have attracted considerable attention in recent years, as they have been found to be involved in many physiological processes, including membrane synthesis and transport, protein...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00C01B32/15G01N21/64
CPCB82Y20/00B82Y40/00C09K11/65C01B32/15G01N21/6486
Inventor 范红松高东刘阿敏张雨生
Owner SICHUAN UNIV
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