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Methane-oxidizing bacteria separated from dairy cow feces and separation method thereof

A technology of methane-oxidizing bacteria and cow manure, applied in the biological field, can solve the problems of animal poisoning, death, non-use, etc.

Inactive Publication Date: 2016-08-17
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chloral hydrate is converted into chloroform in the rumen, and chloroform can reduce the generation of CH4, but chloral hydrate damages the liver, and long-term feeding can cause animal poisoning and even death, so it is not used in actual production

Method used

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  • Methane-oxidizing bacteria separated from dairy cow feces and separation method thereof
  • Methane-oxidizing bacteria separated from dairy cow feces and separation method thereof
  • Methane-oxidizing bacteria separated from dairy cow feces and separation method thereof

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Embodiment Construction

[0048] The bacterial strain of the present invention has the preservation number of the General Microorganism Center of China Microorganism Culture Collection Management Committee as CGMCCNo.11836.

[0049] Preservation unit: General Microbiology Center of China Committee for Microorganism Culture Collection

[0050] Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing

[0051] Deposit date: December 7, 2015

[0052] Deposit number: CGMCC No.11836

[0053] Classification name: Methylobacterium sp.

[0054] The active ingredient of the bacterial agent of the present invention is a methanotrophic bacteria expression strain isolated from cow feces, CGMCC No. 11836.

[0055] The methanotrophs isolated from the cow feces of the present invention have a medium composition in parts by weight:

[0056] Liquid A: KH 2 PO 4 0.8~1.1, Na 2 HPO 4˙ 12H 2 O 2.8~3.0, MgSO 4˙ 7H 2 O 0.30~0.34, (NH 4 ) 2 SO 4 2 2.8~3.2;

[0057] Trace element B solution: Mg...

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Abstract

The invention discloses methane-oxidizing bacteria separated from dairy cow feces and a separation method thereof, and belongs to the field of biotechnology. The invention aims to provide the methane-oxidizing bacteria separated from the dairy cow feces and the separation method thereof. The strain has the preservation number of China General Microbiological Culture Collection Center of CGMCC No.11836. The methane-oxidizing bacteria have the beneficial effects that the methane-oxidizing bacteria can be grown with greenhouse gas methane as a carbon source, and has a certain effect on reducing methane production, namely reducing the greenhouse effect; the methane-oxidizing bacteria can degrade halogenated hydrocarbon compounds, and has important application value in nature carbon cycling and industrial biotechnology and environmental governance; and the methane-oxidizing bacteria are used as a main ingredient for preparation of probiotic preparations to be applied in clinic, and plays an important role in methane waste gas emission caused by ruminant rumen fermentation and the waste of animal intake energy.

Description

technical field [0001] The invention belongs to the field of biotechnology. Background technique [0002] With the increasing intensity of the greenhouse effect, research on greenhouse gases has attracted widespread attention from scholars from all over the world. Methane is a greenhouse gas second only to carbon dioxide. Studies have shown that methane (CH 4 ) of the greenhouse effect is carbon dioxide (CO 2 ) 20 to 30 times that of global warming, accounting for 15% to 20% of global warming. Ruminants account for 97 percent of global methane emissions from livestock, while cattle (excluding buffalo) account for about 75 percent. In my country, the amount of methane emitted by animal gastrointestinal fermentation accounts for 29.7% of the total methane emissions. According to research, usually 6% to 10% of the intake of dairy cows in production can always be converted into methane, which is emitted into the atmosphere in the form of belching. Methane emissions from ru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12R1/01
CPCC12N1/02C12N1/20C12N1/205C12R2001/01
Inventor 刘国文刘欢李心慰王哲李小兵赵晨旭
Owner JILIN UNIV
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