Method for producing polypeptide enramycin with zymotechnics

Abstract

The invention relates to a method for producing polypeptide antibiotics enramycin by the fermentation method, which pertains to the fields of agricultural biotechnology, industrial biotechnology and the fermentation engineering. The method is as follows: (1) slant culture: a Streptomyces sp.NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days; (2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days; (3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.

Description

technical field

[0001] The invention relates to a fermentation method for producing polypeptide antibiotic amellamycin, which belongs to the fields of agricultural biotechnology, industrial biotechnology and fermentation engineering. Background technique

[0002] It has been nearly 60 years since antibiotics were added to feed to promote the growth and development of livestock and improve feed. Many countries have used antibiotic additives for a long time. However, with the widespread use of antibiotic growth promoters, a series of problems have gradually emerged, mainly drug residues and drug resistance. The long-term, extensive and indiscriminate use of antibiotic feed has brought great trouble and difficulty to the control and treatment of bacterial diseases, and also brought potential threats to human health. Although there are many controversies about whether antibiotics should be used in feed, practice has proved that rational use of antibiotic additives can promote t...