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Recombinant Pichia pastoris expressing glucose oxidase fermentation device and fermentation culture method

A technology of glucose oxidase and Pichia pastoris, which is applied in the direction of oxidoreductase, biochemical cleaning device, biochemical equipment and methods, etc., can solve the problems of inability to supplement nutrient salt regulation, unfavorable cell growth, and inability to monitor nutrition on-line in real time Salt changes and other issues

Pending Publication Date: 2018-09-18
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

[0002] At present, during the fermentation process of Pichia pastoris G / GMH1, the nutrient salt is added at one time, which may easily lead to too high or too low salt concentration, which is not conducive to the growth of the bacteria. control

Method used

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  • Recombinant Pichia pastoris expressing glucose oxidase fermentation device and fermentation culture method
  • Recombinant Pichia pastoris expressing glucose oxidase fermentation device and fermentation culture method
  • Recombinant Pichia pastoris expressing glucose oxidase fermentation device and fermentation culture method

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Embodiment Construction

[0025] The specific implementation of the recombinant Pichia pastoris expression glucose oxidase fermentation device and the fermentation culture method of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0026] See attached figure 1 The recombinant Pichia pastoris expression glucose oxidase fermentation device comprises a fermenter 1, a nutrient feed bottle 2 and a control cabinet 3, the fermenter 1 is filled with a Pichia fermentation base liquid, and the fermenter 1 is provided with an agitator 4, and the nutrient The salt feeding bottle 2 is connected to the fermenter 1 through the feeding pump 5, and the living cell electrode 6 is installed in the fermenting tank 1. The control cabinet 3 is connected to the feeding pump 5 and the living cell electrode 6, and the control cabinet 3 transmits 6 times according to the living cell electrode. The electrical conductivity data controls the rotational speed of feeding pump 5 ...

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Abstract

The invention relates to the field of industrial biotechnology, and discloses a recombinant Pichia pastoris expressing glucose oxidase fermentation device, comprising a fermenting tank, a nutrient salt feeding bottle and a control cabinet, wherein the fermenting tank is filled with Pichia astoris fermentation broth. The nutrient salt feeding bottle is communicated with the fermenting tank througha feed pump, and the fermenting tank is internally provided with a living cell electrode; the control cabinet is connected with the feed pump and the living cell electrode, and the control cabinet controls the feed pump according to the conductivity data returned by the living cell electrode to feed. The invention also discloses a culturing method of Pichia pastoris, comprising the steps of glycerol batch culture, glycerol fed-batch culture and methanol induction. According to the device and method provided by the invention, the nutrient salt concentration of a culture medium can be monitoredin real time by the living cell electrode, so that the Pichia pastoris G / GMH1 is in an optimal production state, thereby improving the expression efficiency of bacteria expressing glucose oxidase.

Description

technical field [0001] The invention relates to the field of industrial biotechnology, in particular to a fermentation device for expressing glucose oxidase in recombinant Pichia pastoris and a fermentation culture method. Background technique [0002] At present, during the fermentation process of Pichia pastoris G / GMH1, the nutrient salt is added at one time, which may easily lead to too high or too low salt concentration, which is not conducive to the growth of the bacteria. regulation. Contents of the invention [0003] The purpose of the present invention is to solve the above problems, to provide a recombinant Pichia pastoris expression glucose oxidase fermentation device and fermentation culture method, real-time monitoring of the nutrient salt concentration of the culture medium through living cell electrodes, so that Pichia pastoris G / GMH1 is at the optimum Excellent production status, thereby improving the expression efficiency of the bacterial cell expressing g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/42C12M1/02C12N9/04C12N1/16C12R1/84
CPCC12M21/14C12M27/02C12M35/02C12N1/16C12N9/0006C12Y101/03004
Inventor 钱江潮吴凡王泽建张文玉庄英萍储炬
Owner EAST CHINA UNIV OF SCI & TECH
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