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Compound of keratinase and application in industrial production

A technology of keratinase and compound enzyme, applied in the field of industrial biology, can solve the problems of leather collagen composition or integrity damage, etc., and achieve the effects of complete hair removal effect, good application prospect and good application prospect.

Active Publication Date: 2018-04-17
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the choice of keratinase with low collagen or no collagen activity to apply to the leather depilation process has the characteristics of cleanliness, environmental friendliness, thorough depilation, high action efficiency, high leather integrity, and good quality. However, in the current grid industry There is no collagen-free keratinase that can be applied industrially, and all existing keratinases will damage the collagen composition or integrity of leather to varying degrees

Method used

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  • Compound of keratinase and application in industrial production
  • Compound of keratinase and application in industrial production
  • Compound of keratinase and application in industrial production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Application characteristics of embodiment 1 keratinase KERBP

[0029] (1) Optimum pH and pH stability

[0030] The optimal reaction pH of KERBP is 8.0. With the continuous increase of pH, the enzyme activity of keratinase first increases and then decreases; the stable pH range of recombinant keratinase is 7.0-9.0, and the residual enzyme activity in this range is >75% , at pH11.0, the residual enzyme activity decreased rapidly. The results showed that after incubation for 1 hour in the pH range of 8.0-10.0, the enzyme activity was still high, indicating that the recombinant keratinase was more stable in the alkaline pH range. In the tanning process, the operating conditions of multiple production processes such as soaking, dehairing and liming are often alkaline, while the optimum reaction pH of recombinant keratinase is alkaline, which is suitable for the tanning process.

[0031] (2) Effect of chemical reagents on keratinase activity

[0032] Under the reaction con...

Embodiment 2

[0046] Embodiment 2 Recombinant keratinase is applied to the depilation of sheepskin

[0047] The amount of keratinase is 10000U / 50mL, mixed with raw sheepskin of about 6cm×6cm size (equivalent to 278U / cm2 raw sheepskin), and shaken and reacted at 37°C and 200rpm. A commercial depilatory protease derived from Bacillus was selected as a positive control for enzymatic hair removal, and its dosage was the same as that of the recombinant keratinase KERBP in the hair removal method.

[0048] The above treatment results show that without using heavily polluted chemical reagents such as sulfide, keratinase and sheepskin can be completely depilated after 9 hours of co-incubation. The wool was not removed when the negative control was mixed with water and sheepskin; compared with the sheepskin depilated by chemical method, the color was heavier and the touch was harder, and the sensory test showed that the sheepskin depilated by recombinant keratin was lighter in color, smoother and so...

Embodiment 3

[0050] The depilatory effect under the different conditions of embodiment 3

[0051] The hair removal process mainly includes degrading the epidermis, hydrolyzing the intercellular mucin in the dermal hair follicle, damaging the link between the hair root and the hair follicle, causing the cells to separate from each other, weakening the root sheath and losing the wrapping effect on the hair root, and then the hair can be restored. detach from the hair follicle. Therefore, in this process, the enzymes in the skin must first penetrate into the junction of the epidermis until the dermis reaches the junction with the root sheath, hair papilla and hair bulb to achieve the depilatory effect. Adopt the addition amount of table 1 to carry out depilation. Select lipase, put about 6 cm×6 cm raw sheepskin into the compound enzyme solution (50 mL), shake and react at 37°C and 200 rpm.

[0052] Through the sensory evaluation of the hair removal process and hair removal effect, the resul...

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Abstract

The invention discloses a compound of keratinase and application in industrial production, and belongs to the field of the industrial biotechnology. The invention provides a method in which keratinaseis independently applied to the industrial tanning depilation or is compounded with lipase and amylase to be applied to the industrial tanning depilation, and the beneficial effects that depilation efficiency is high, the action conditions are mild, and the safety performance is good are achieved. The keratinase is adopted and can be used for independently completing sheepskin depilation, the quality of depilated leather is subjected to representation, and the depilation effect is the same as that of commercial enzyme; besides, lipase, amylase and keratinase are provided to perform compound depilation on sheepskin, the three kinds of enzymes are combined to have a collaborative effect on sheepskin depilation, the using amount of the enzymes can be reduced, and the depilation efficiency isimproved. The keratinase and a compounded enzyme preparation of the keratinase have good application prospects in the tanning field.

Description

technical field [0001] The invention relates to a compound of keratinase and its application in industrial production, belonging to the field of industrial biotechnology. Background technique [0002] The depilation process in the leather production process is an essential step, but the depilatory agent used in traditional chemical depilation is sulfide (Na 2 S) and lime. However, the depilatory waste liquid contains high concentration of sulfides, which is also the main link of environmental pollution in the leather industry. In recent years, the use of protease for hair removal has received a lot of attention, but the quality of protease is unstable and can often degrade collagen, so the specificity is poor and the action is complicated. Therefore, problems such as incomplete hair removal or rotten and loose surfaces are prone to occur during production. It is difficult to control, which is also the main reason why enzymatic hair removal cannot fully replace chemical met...

Claims

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Application Information

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IPC IPC(8): C14C1/06C12N9/50C12N9/26C12N9/20
Inventor 史劲松龚劲松张荣先许正宏李恒苏畅
Owner JIANGNAN UNIV
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