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Method for synthesizing natamycin through solid-state fermentation of industrial and agricultural byproducts

A technology of natamycin and solid-state fermentation, which is applied in the field of industrial biology, can solve the problems that the inherent defects of liquid submerged fermentation cannot be effectively solved, the concentration of natamycin products is low, and the pressure on environmental protection of enterprises is increased, and energy consumption can be achieved. Reduced power consumption, low power consumption, and no pollution

Active Publication Date: 2019-02-12
山东元泰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above studies have greatly improved the biosynthesis level of natamycin, however, the inherent defects of liquid submerged fermentation have not been effectively resolved
First, liquid fermentation requires aeration and high-speed stirring for 5-7 consecutive days, which consumes a lot of energy; second, the substrates used for natamycin liquid fermentation mainly include glucose, starch, soybean peptone, beef extract, yeast Extract, raw material cost is higher; Third, the concentration of natamycin product in liquid fermentation is low (2-14g / L), will produce a large amount of organic waste water after fermentation finishes, and then increases the environmental protection pressure of enterprise

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A 250mL triangular flask was used as a fermentation vessel, 10g of bran, rice bran, bagasse or wheat straw was used as a carbon source, 5g of rapeseed cake was used as a nitrogen source, 0.45g of rice husk was used as a fluffy carrier, and 0.45g of industrial glycerin was used as an auxiliary carbon source. Configure 16g of dry weight solid-state fermentation medium. Add 70% (v / w, g / L, where w is the total mass of carbon source, nitrogen source, auxiliary carbon source and fluffy carrier, the same below) water to the solid-state fermentation medium, and autoclave at 126°C Sterilize in the pot for 30min. Inoculate the 42-52h seed culture solution of Streptomyces luteus Z28 with an inoculum amount of 15% (v / w) in the above medium, and place it in a constant temperature incubator at 28°C for fermentation and cultivation for 7 days. Turn over twice to ensure oxygen supply, and the final natamycin production concentration reaches 9.53, 3.04, 6.10, 2.06 mg·g -1 Dry weight (...

Embodiment 2

[0053] Use a 250mL triangular flask as a fermentation vessel, 10g of bran as a carbon source, 5g of rapeseed cake, soybean cake, rice distiller's grains or oyster mushroom residue as a nitrogen source, 0.45g of rice husk as a fluffy carrier, and 0.45g of industrial glycerin as an auxiliary Carbon source, configure 16g dry weight solid fermentation medium. Add 70% (v / w) water to the solid-state fermentation medium, and sterilize in an autoclave at 126° C. for 30 minutes. Inoculate the 42-52h seed culture solution of Streptomyces luteus Z28 with an inoculum amount of 15% (v / w) in the above medium, and place it in a constant temperature incubator at 28°C for fermentation and cultivation for 7 days. Turn over twice to ensure oxygen supply, and the final natamycin production concentration reaches 9.53, 6.96, 1.56, 3.34 mg·g -1 dry weight.

Embodiment 3

[0055] Use a 250mL triangular flask as the fermentation container, the mass ratio of carbon source and nitrogen source is 3:1, 2:1, 1:1, 1:2, 1:3, and the total mass of carbon source and nitrogen source is 15g, 0.45g rice husk It is a fluffy carrier, 0.45g of industrial glycerin is used as an auxiliary carbon source, and 16g of dry weight solid fermentation medium is configured. Add 70% (v / w) water to the solid-state fermentation medium, and sterilize in an autoclave at 126° C. for 30 minutes. Inoculate the 42-52h seed culture solution of Streptomyces luteus Z28 with an inoculum amount of 15% (v / w) in the above medium, and place it in a constant temperature incubator at 28°C for fermentation and cultivation for 7 days. Turn over twice to ensure oxygen supply, and the final natamycin production concentration reaches 2.32, 9.53, 4.51, 1.80, 1.13 mg·g -1 dry weight.

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Abstract

The invention discloses a method for synthesizing natamycin through solid-state fermentation of industrial and agricultural byproducts, and in particular relates to a method for synthesizing the natamycin by taking cheap agricultural and light industrial byproducts as substrates through a solid-state fermentation method, and belongs to the technical field of industrial biotechnologies. A solid-state fermentation culture medium provided by the invention comprises a carbon source, a nitrogen source, an auxiliary carbon source and a fluffy carrier, wherein the mass ratio of the carbon source to the nitrogen source is (1 to 3) to (1 to 3); the adding amount of the auxiliary carbon source is 2 to 5 weight percent; the adding amount of the fluffy carrier is 2 to 5 weight percent; the water content in the culture medium is 50 to 100 percent (v / w); the solid-state fermentation medium provided by the invention is used for culturing streptomyces gilvosporeus and fermenting to produce the natamycin, so that the energy consumption and cost in a fermentation process are greatly reduced; meanwhile, the industrial and agricultural byproducts are effectively utilized.

Description

technical field [0001] The invention relates to a method for synthesizing natamycin by solid-state fermentation of industrial and agricultural by-products, in particular to a method for synthesizing natamycin by solid-state fermentation using cheap agricultural and light industrial by-products as substrates, belonging to field of industrial biotechnology. Background technique [0002] Natamycin is a secondary metabolite synthesized by fermentation of Streptomyces. (Chen et al., J.Agr.FoodChem., 2008), Streptomyces lydides (Lu et al., Braz.J.Microbiol., 2008) and Streptomyces natalis (Aparicio et al., J.Biol Chem., 1999; El-Enshasy et al., J. Basic. Microb., 2000). Natamycin is a broad-spectrum antifungal agent with a polyene macrolide structure, which has a strong inhibitory effect on mold and yeast. In addition, it has been designated as a "generally safe" (GRAS) compound by the US Food and Drug Administration (FDA), which greatly expands its application range. At prese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12N1/20C12R1/465
CPCC12N1/20C12P19/62
Inventor 曾昕李峰曾化伟徐大勇张标信丙越
Owner 山东元泰生物工程有限公司
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