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Heat-resistant Yarrowia Lipolytica and application thereof

A technology of Yarrow lipolytic yeast and lipolytic yeast, applied in fermentation, microorganisms, fungi and other directions, to achieve the effect of reducing dosage and investment

Active Publication Date: 2019-07-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no high temperature tolerant Yarrow lipolytica available

Method used

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  • Heat-resistant Yarrowia Lipolytica and application thereof
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  • Heat-resistant Yarrowia Lipolytica and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Plate growth

[0028] Yarrowia Lipolytica BBE-18 is obtained by mutagenesis on the basis of the original strain Yarrowia Lipolytica BBE-17.

[0029] YPD medium: glucose 20g / L, peptone 20g / L, yeast powder 10g / L.

[0030] Single colonies of Yarrow lipolytic yeast BBE-18 and Yarrow lipolytic yeast BBE-17 were picked and inoculated into YPD liquid medium, and cultured at 35°C and 30°C for 20h. Then take the dilution factor as 10 0 , 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 10μL of the bacteria solution was spot-coated on the YPD plate. Because the temperature tolerance of yeast on the plate is inferior to that of liquid medium, the plate was placed in an incubator at 33°C for cultivation, and the colony was observed after 3 days.

[0031] The results show (see figure 1 ), at 33℃, BBE-17 can grow rapidly with the advantage of the flora when the bacteria concentration is high; however, with dilution, the bacteria concentration decreases and the survival rate at 33℃ decrease...

Embodiment 2

[0032] Example 2: Low-temperature fermentation of Yarrow lipolytic yeast

[0033] (1) Seed culture: pick single colonies of Yarrow lipolytic yeast BBE-17 and BBE-18, respectively, and inoculate them in 4mL YPD liquid medium, and shake culture for 20h in a shaker at 30℃ and 220 rpm to obtain sub Rhodiola lipolytica BBE-17, BBE-18 seed liquid.

[0034] (2) Shake flask fermentation culture: respectively take the Yarrow lipolytic yeast BBE-17 and BBE-18 seed solution in step (1), and inoculate them into the fermentation medium according to the inoculum amount of 5%. Shake culture in a shaker at 220 rpm.

[0035] Fermentation medium: glucose 120g / L, yeast powder 10g / L, ammonium citrate 5g / L, magnesium sulfate 0.5g / L, potassium dihydrogen phosphate 0.25g / L.

[0036] The results showed that: when the fermentation time was 64h, the erythritol output of thermotolerant Yarrowia lipolytic yeast BBE-18 reached 44.7g / L, and the conversion rate of glucose to erythritol was 66%; the fermentation ti...

Embodiment 3

[0037] Example 3: High-temperature fermentation of Yarrow lipolytic yeast

[0038] The fermentation conditions are the same as those in Example 2, except that the fermentation temperature is adjusted to 35°C, and the glucose concentration in the fermentation medium is increased to 150 g / L. The specific fermentation process is as follows:

[0039] (1) Seed culture: pick single colonies of Yarrow lipolytic yeast BBE-17 and BBE-18, respectively, and inoculate them in 4mL YPD liquid medium, and shake culture for 20h in a shaker at 30℃ and 220 rpm to obtain sub Rhodiola lipolytica BBE-17, BBE-18 seed liquid.

[0040] (2) Shake flask fermentation culture: respectively take the Yarrow lipolytic yeast BBE-17 and BBE-18 seed solution in step (1), and inoculate them into the fermentation medium according to the inoculation amount of 5%. Shake culture in a shaker at 220 rpm.

[0041] Fermentation medium: glucose 150g / L, yeast powder 10g / L, ammonium citrate 5g / L, magnesium sulfate 0.5g / L, potas...

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Abstract

The invention discloses heat-resistant Yarrowia Lipolytica and application thereof and belongs to the technical field of industrial biology. The heat-resistant Yarrowia Lipolytica is preserved in China Center for Type Culture Collection which is located in Wuhan University, Wuhan, China on March 18th, 2019, and the preservation number of the heat-resistant Yarrowia Lipolytica is CCTCC No. M2019163. The heat-resistant Yarrowia Lipolytica BBE-18 can ferment at 35 DEG C to produce erythritol, the erythritol yield of the heat-resistant Yarrowia Lipolytica BBE-18 reaches 68.1g / L when the fermentation time is 80 hours, and the erythritol conversion rate of glucose is 67%. The heat-resistant Yarrowia Lipolytica has certain industrialization potential and provides possibility for fermentation cooling cost lowering and summer production halt avoiding.

Description

Technical field [0001] The invention relates to a heat-resistant Yarrow lipolytic yeast and its application, and belongs to the field of industrial biotechnology. Background technique [0002] Yarrowia Lipolytia (Yarrowia Lipolytia) is widely regarded as safe. It has passed the GRAS (generally regarded assafe) certification of the Food and Drug Administration (FDA) and can be widely used in the food industry. Its characteristics are described in detail in the patent CN201710540443.3 (publication date: 2017.09.15). The fermentation temperature of Yarrow lipolytic yeast is generally 28℃~30℃. It belongs to aerobic fermentation microorganisms. During the fermentation process, it will produce a lot of biological heat. There are few reports of high temperature resistant Yarrow lipolytic yeast. To control the fermentation temperature to 28-30℃ in the industrial production process, a large amount of cooling water needs to be consumed, and the cooling coils of large-scale fermentation ta...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/18C12R1/645
CPCC12N1/16C12P7/18C12N1/145C12R2001/645
Inventor 张娟邱学良李江华堵国成陈坚朱政明
Owner JIANGNAN UNIV
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