High-yield paenibacillus amyloliquefaciens csuft F14 for chitin deacetylase and its application

A technology of amylolytic spores and deacetylation, applied in the field of microorganisms, can solve the problems of immature application of technology, large consumption of raw materials and energy, and many operating steps, and achieve good development and application prospects, easy industrial production, and simple cultivation conditions Effect

Inactive Publication Date: 2009-09-30
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chitosan is mainly produced by the concentrated alkali method at home and abroad, but there are many problems in its preparation process, such as: not completely removing protein and calcium salts, low decaproylation degree, low viscosity,

Method used

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  • High-yield paenibacillus amyloliquefaciens csuft F14 for chitin deacetylase and its application
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  • High-yield paenibacillus amyloliquefaciens csuft F14 for chitin deacetylase and its application

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Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1: the screening method of bacterial strain of the present invention

[0022] 1) Plate primary screening

[0023] The flat coating method is used. Weigh 10g of the soil sample collected from the banks of the Xiangjiang River, pour it into 90mL of sterile water containing glass beads, shake it fully for 20min, and pipette 1mL of gradient dilution to 10 -6 For double concentration, take 1ml and spread it on the petri dish added with PDA medium; at the same time, take 1ml and spread it on the petri dish added with beef extract peptone medium. The PDA culture is based on 26°C for 4 days, and the beef extract peptone culture is based on 30°C for 2 days. Pick a single colony from the culture medium and continue to purify. Repeat 3 times. That is, fully purified. Pick a single colony from the last purification plate and inoculate it on the slant medium for culture and preservation for future use.

[0024] Select the obtained slant strain after separation and pur...

Embodiment 2

[0036] Embodiment 2: the application of bacterial strain of the present invention

[0037] Chitin is added to the fermentation medium (medium components: chitin (greater than 120 mesh) 1.5%, bean cake powder 2.0%, corn flour 2%, glucose 2%, NaNO 3 0.2%, K 2 HPO 4 0.1%, KH 2 PO 4 0.1%, MgSO 40.05%), 36°C, 160rpm / min, cultivated for 2 days, added 10% NaOH solution after filtering the fermentation broth, stirred while adding, until the pH value was 10-11, white flocculent precipitate appeared, centrifuged after standing, and The precipitate is washed with distilled water until the pH value is neutral, centrifuged, collected, and dried to obtain a chitosan product.

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PUM

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Abstract

The present invention discloses high-yield paenibacillus amyloliquefaciens csuft F14 for chitin deacetylase and the application of the paenibacillus amyloliquefaciens csuft F14. The preservation entity of the strain is CCTCC, and the preservation number is CCTCC M 209062. The advantage of the present invention is: the strain can be cultured simply and has high enzyme activity when it is used to produce chitin deacetylase, and is favorable for development and utilization of chitin deacetylase, and has excellent application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacterium with high chitin deacetylase production and application thereof. Background technique [0002] Chitin, also known as chitin, chitin, etc., its chemical name is poly[(1,4)-2-acetylamino-2-deoxy-β-D-glucose], is a natural polymer polysaccharide, but its dissolution The performance is very poor, so its application is limited. Chitosan (chitosan) is the product of partial or complete removal of the acetyl group in the chitin molecule, and its solubility has been greatly improved. Chitosan is widely used at home and abroad. At present, it has been widely used in food, medicine, chemical industry, biology, textile, environmental protection, functional materials and other fields. For example, in the pharmaceutical industry, artificial skin and drug carriers can be produced. It is used as dyeing agent and color fixing agent in light textile industry. I...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C12R1/07
Inventor 章怀云周国英刘高强刘君昂李河何苑皞宋光桃李琳
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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