Paenibacillus and its application in water body purification
A technology of Paenibacillus and Bacillus, applied in the field of Paenibacillus and its application, can solve the problems of heavy workload and high cost, and achieve the effects of strong adaptability, good microbial growth, effective and stable treatment
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Embodiment 1
[0037] Screening and Identification of Example 1 Bacterial Strains
[0038] 1. Screening
[0039] 1) Add 0.3g of yeast powder to 100mL of the Paenibacillus-containing mud sample, shake gently at 30°C at 100 rpm for enrichment and culture for 1 week, and use a microscope to detect the growth of the bacteria; the mud sample is taken from Longjiang River, Guangxi. Bacteria grow until the absorbance value of the bacterial culture solution at 600nm is measured, and if the obtained OD600 value is between 0.6 and 0.8.
[0040] 2) Filter the bacteria in the above culture solution by membrane filtration, and wash the bacteria with 20 mL of sterile water. Inoculate multiple 100mL enrichment media with 5% inoculum amount, culture at 45°C, and set no inoculation control (CK). The growth of bacteria was observed after two weeks of culture on a shaker at 100rpm.
[0041] 3) Gradually dilute the enriched culture medium by 1, 2, 3, 4, 5 (corresponding to 10 -1 , 10 -2 , 10 -3 , 10 -4 ,...
Embodiment 2
[0050] Embodiment 2 purifying water body
[0051] 1. Pass the typical black and odorous water body (the black and odorous water body of Lashan River in Jinan) into the biological oxidation reactor, which is filled with commercially available silicon-based porous fillers.
[0052] The wastewater quality is as follows: pH7.0; chemical oxygen demand (COD), 45 mg / L; total nitrogen (TN), 15 mg / L, total phosphorus (P), 3 mg / L.
[0053] 2. Inoculate the identified Paenibacillus strains in the enrichment medium and cultivate them on a shaker at 100 rpm at 25°C until the bacterial concentration is 10 8 Individual / mL; then inoculate the bacterium liquid (also can be referred to as bacterium agent, with the bacterium agent described in summary of the invention) in the biological oxidation reactor, the inoculation amount is 3L / m 3 . The bottom of the biooxidation reactor is filled with air, the aeration rate varies according to the water quality, and the hydraulic retention is 1 hour; ...
Embodiment 3
[0058] Purification of water body at low temperature in embodiment 3
[0059] 1. Pass the typical black and odorous water body (the black and odorous water body of Lashan River in Jinan) into the biological oxidation reactor, which is filled with commercially available silicon-based porous fillers. The water quality of the wastewater is as follows: pH7.0; chemical oxygen demand ( COD), 45mg / L; total nitrogen (TN), 15mg / L, total phosphorus (P), 3mg / L.
[0060] 2. Inoculate the identified Paenibacillus species into the enrichment medium, and culture it on a shaker at 5°C at 100 rpm until the bacterial concentration is 10 8 Individual / mL; then inoculate the bacterial solution (also called the bacterial agent, the same as the bacterial agent described in the summary of the invention) into the low-temperature environment (3 . The bottom of the biooxidation reactor is filled with air, the aeration rate varies according to the water quality, and the hydraulic retention is 1 hour;
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