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Paenibacillus and its application in water body purification

A technology of Paenibacillus and Bacillus, applied in the field of Paenibacillus and its application, can solve the problems of heavy workload and high cost, and achieve the effects of strong adaptability, good microbial growth, effective and stable treatment

Active Publication Date: 2017-02-15
重庆华悦生态环境工程研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microbial preparation depends on the composition of various microorganisms to function, and it needs to separate, screen and enrich the above-mentioned microorganisms for preparation, which requires a large workload and high cost.

Method used

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  • Paenibacillus and its application in water body purification
  • Paenibacillus and its application in water body purification
  • Paenibacillus and its application in water body purification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Screening and Identification of Example 1 Bacterial Strains

[0038] 1. Screening

[0039] 1) Add 0.3g of yeast powder to 100mL of the Paenibacillus-containing mud sample, shake gently at 30°C at 100 rpm for enrichment and culture for 1 week, and use a microscope to detect the growth of the bacteria; the mud sample is taken from Longjiang River, Guangxi. Bacteria grow until the absorbance value of the bacterial culture solution at 600nm is measured, and if the obtained OD600 value is between 0.6 and 0.8.

[0040] 2) Filter the bacteria in the above culture solution by membrane filtration, and wash the bacteria with 20 mL of sterile water. Inoculate multiple 100mL enrichment media with 5% inoculum amount, culture at 45°C, and set no inoculation control (CK). The growth of bacteria was observed after two weeks of culture on a shaker at 100rpm.

[0041] 3) Gradually dilute the enriched culture medium by 1, 2, 3, 4, 5 (corresponding to 10 -1 , 10 -2 , 10 -3 , 10 -4 ,...

Embodiment 2

[0050] Embodiment 2 purifying water body

[0051] 1. Pass the typical black and odorous water body (the black and odorous water body of Lashan River in Jinan) into the biological oxidation reactor, which is filled with commercially available silicon-based porous fillers.

[0052] The wastewater quality is as follows: pH7.0; chemical oxygen demand (COD), 45 mg / L; total nitrogen (TN), 15 mg / L, total phosphorus (P), 3 mg / L.

[0053] 2. Inoculate the identified Paenibacillus strains in the enrichment medium and cultivate them on a shaker at 100 rpm at 25°C until the bacterial concentration is 10 8 Individual / mL; then inoculate the bacterium liquid (also can be referred to as bacterium agent, with the bacterium agent described in summary of the invention) in the biological oxidation reactor, the inoculation amount is 3L / m 3 . The bottom of the biooxidation reactor is filled with air, the aeration rate varies according to the water quality, and the hydraulic retention is 1 hour; ...

Embodiment 3

[0058] Purification of water body at low temperature in embodiment 3

[0059] 1. Pass the typical black and odorous water body (the black and odorous water body of Lashan River in Jinan) into the biological oxidation reactor, which is filled with commercially available silicon-based porous fillers. The water quality of the wastewater is as follows: pH7.0; chemical oxygen demand ( COD), 45mg / L; total nitrogen (TN), 15mg / L, total phosphorus (P), 3mg / L.

[0060] 2. Inoculate the identified Paenibacillus species into the enrichment medium, and culture it on a shaker at 5°C at 100 rpm until the bacterial concentration is 10 8 Individual / mL; then inoculate the bacterial solution (also called the bacterial agent, the same as the bacterial agent described in the summary of the invention) into the low-temperature environment (3 . The bottom of the biooxidation reactor is filled with air, the aeration rate varies according to the water quality, and the hydraulic retention is 1 hour;

...

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Abstract

The invention relates to a water body purifying microorganism, and particularly discloses a paenibacillus YE-1 and its application. The paenibacillus YE-1 is preserved at China General Microbiological Culture Collection Center, the preservation date is August 4, 2016 and the preservation number is CGMCC No. 12814. The paenibacillus YE-1 can be used for effectively absorbing degrading organic matters in black and odorous waste in a biological oxidation reactor, and purifying water quality. Furthermore, since a distributed photovoltaic system supplies power energy to an aeration device of the biological oxidation reactor, the technical flow needs not additional power energy, the operation cost is low, and the treatment is effective and stable.

Description

technical field [0001] The invention relates to microorganisms for purifying water bodies, in particular to a Paenibacillus and application thereof. Background technique [0002] With the rapid development of economy and the acceleration of urbanization, the problems of water quality pollution and ecological degradation of rivers in many cities in my country are very prominent, and even seasonal and perennial black and odorous water bodies have appeared. The existing black and odorous water treatment methods mainly adopt plant purification and aeration treatment, the purification load is low, the treatment process requires additional power input, and the operating cost is high. [0003] Bacillus is the dominant population in the soil. It can strongly decompose carbon, nitrogen, phosphorus, and sulfur pollutants, and can decompose proteins and complex polysaccharides. It also plays an important role in the decomposition of water-soluble organic matter. At the same time, it ca...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C02F2101/30C12N1/205C12R2001/01
Inventor 任南琪杨平叶文华刘兴宇
Owner 重庆华悦生态环境工程研究院有限公司
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