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Microbial compound bacteria for preventing and treating citrusyellowshoot and preparation method and application thereof

A microbial compound bacteria and microorganism technology are applied in the field of microbial compound bacteria for preventing and treating citrus Huanglongbing and its preparation, which can solve the problems of treating symptoms but not the root cause, and failing to solve the problem from the depth of the pathogen, and achieve the prevention and control of Huanglongbing and significant Huanglongbing. Effect

Active Publication Date: 2016-04-13
江西啄木蜂科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods "treat the symptoms but not the root cause" and do not solve the problem from the depth of the pathogen. At present, the existing technology lacks a feasible method that can effectively directly reduce or eliminate the pathogen in the diseased plant

Method used

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  • Microbial compound bacteria for preventing and treating citrusyellowshoot and preparation method and application thereof
  • Microbial compound bacteria for preventing and treating citrusyellowshoot and preparation method and application thereof

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preparation example Construction

[0035] When what the present invention provides is microbial complex bacterial liquid, the present invention provides a kind of preparation method of microbial complex bacterial liquid, comprises the following steps:

[0036] a1) fermentation of Paenibacillus Kliben;

[0037] a2) fermentation of Bacillus subtilis;

[0038] a3) mixing of fermentation broth;

[0039] There is no time sequence limitation between step a1) and step a2).

[0040] In the invention, the Paenibacillus klibben is fermented to obtain the Paenibacillus klibben fermentation liquid. In the present invention, described Paenibacillus klibben fermentation is specifically:

[0041] The Paenibacillus species were activated for culture;

[0042] Inoculating the activated cultured bacteria into a liquid medium for liquid fermentation to obtain Paenibacillus kleben fermentation liquid.

[0043] In the present invention, it is preferred to inoculate the preserved Paenibacillus species in the nutrient agar medium ...

Embodiment 1

[0069] The preparation of Paenibacillus Kliben fermented liquid, the Paenibacillus Klibben is inoculated in the liquid culture after being sterilized for 35min at 121 DEG C after expanded culture, and described liquid culture medium composition is 30 weight parts of sucrose, 1.5 weight parts of sodium nitrate 1.0 parts by weight of potassium dihydrogen phosphate, 0.5 parts by weight of magnesium sulfate, 0.5 parts by weight of potassium chloride, and 0.01 parts by weight of iron sulfate. Fermentation in a fermenter, fermentation conditions: temperature 30 ° C, pH value 6.9, tank pressure 0.1 MPa, fermentation ventilation maintained at 1 v / v min, time 76 hours, after 72 hours of fermentation, use a microscope every 2 hours The detection method is to detect the total number of viable bacteria in the fermentation broth, and stop the fermentation when the total number of viable bacteria reaches 9.6 billion / ml to obtain the Paenibacillus kleben fermentation broth.

Embodiment 2

[0071] The preparation of Bacillus subtilis fermented liquid, expand Bacillus subtilis and inoculate in the liquid culture after 121 ℃ of sterilization 40min, described liquid culture medium composition is peptone 0.03 weight part, yeast powder 0.03 weight part, sucrose 0.025 weight part , 0.007 parts by weight of ammonium sulfate, 0.003 parts by weight of trisodium citrate, 0.003 parts by weight of potassium dihydrogen phosphate, 0.0005 parts by weight of magnesium sulfate, 0.00005 parts by weight of iron sulfate, and 0.01 parts by weight of manganese sulfate. Fermentation in a fermenter, fermentation conditions: temperature 29°C, pH value 6.8, tank pressure 0.07MPa, fermentation ventilation maintained at 1v / v min, time 36 hours, after 28 hours of fermentation, the fermentation broth was tested every 2 hours The total number of live bacteria, when the total number of live bacteria reaches 9.5 billion / ml, stop the fermentation to obtain the Bacillus subtilis fermentation liquid...

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Abstract

The invention provides microbial compound bacteria, a preparation method thereof and application of the microbial compound bacteria to prevention and treatment of citrusyellowshoot. The microbial compound bacteria comprise Cribben paenibacillus and bacillus subtilis, the biological preservation serial number of the Cribben paenibacillus is CGMCC NO.7996, and the microbial compound bacteria can be specifically microbial compound bacterium liquid or a microbial compound bacterium agent. The preparation method of the microbial compound bacteria includes the specific steps that the Cribben paenibacillus and the bacillus subtilis are mixed in proportion after being fermented. According to the microbial compound bacteria for preventing and treating citrusyellowshoot and the preparation method and application thereof, procreation and propagation of gram negative bacteria are inhibited in a root filling and leaf surface spraying mode, 'bacteria are controlled through bacteria', harmful bacteria in soil are killed, soil quality is optimized, root and stem phloem sieve tubes can be dredged, the problem of nutrient substance conveying barrier is solved, diseased or infected plant nutrients are recovered, growth of new roots is promoted, tree roots are more developed, tree tubular cells are smoother, yellow leaves of fruit trees are turned green, and healthy new tips grow.

Description

technical field [0001] The invention relates to the field of biological control, in particular to a microbial composite bacterium for preventing and treating citrus huanglongbing and its preparation method and application. Background technique [0002] Citrus is the most productive fruit in the world, and it has been cultivated in my country for more than 4,000 years. In 2009, my country's citrus cultivation area and output surpassed Brazil and the United States, ranking first in the world. However, while the citrus industry is developing rapidly, Huanglongbing has become the most serious and devastating disease in citrus production. As early as the middle of the 18th century, there were related reports in India. Reinking reported for the first time in South my country in the 1920s. For more than half a century, citrus huanglongbing has seriously restricted the development of citrus industries in Guangdong, Guangxi, and Fujian. At the end of the 1970s, citrus greening dise...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00A01G13/00C12R1/125C12R1/01
CPCA01G13/00A01N63/00C12N1/20
Inventor 梁小文王根豪严艺波李肖宇曾升华石峥吴俊杰李英武
Owner 江西啄木蜂科技有限公司
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