Soil paenibacillus and application thereof

A bacillus and soil technology, applied in the field of microorganisms, can solve the problems of limiting the amount of gellan gum used and its impact, and achieve the effects of unique properties, increased solution viscosity, and increased solution viscosity

Inactive Publication Date: 2017-02-15
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are certain limiting factors in the application of microbial exopolysaccharides
For example, in order to increase the viscosity of xanthan gum solution, it can only be achieved by increasing the amount of xanthan gum added, but not by simple post-treatment process; there is Ca in the solution 2+ or Mg 2 + Limits the amount of gellan gum used; heat treatments with higher temperatures are affected by thermal gelling

Method used

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  • Soil paenibacillus and application thereof
  • Soil paenibacillus and application thereof
  • Soil paenibacillus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Isolation and identification of Paenibacillus edaphicus NUST16.

[0032] (1) Isolation of Paenibacillus edaphicus NUST16

[0033] The soil Paenibacillus edaphicus NUST16 of the present invention is isolated from coastal brown soil in Yancheng, Jiangsu. The method of separation is:

[0034] ① Screening of bacterial strains: Divide the soil samples into 10 groups, each group weighed 1g of soil samples and added them to the prepared liquid medium, put them in a constant temperature shaking incubator and culture them for 2 days at 200rpm and 30°C for enrichment. Then take 1mL bacterial liquid from each group of Erlenmeyer flasks and reintroduce it into a new liquid medium, culture for 2 days under the same conditions and enrich again. Then take 1 mL of the enriched bacterial solution from each group and add it to 9 mL of sterile water, mix well to make a bacterial suspension, and use 10-fold gradient dilution to take 10 -6 -10 -4 The diluted bacterial suspension was spr...

Embodiment 2

[0042] The cultivation of Paenibacillus edaphicus NUST16 and its application in the production of water-soluble exopolysaccharide.

[0043] Prepare medium, sucrose 5g, peptone 0.5g, NaH 2 PO 4 0.1g, CaCl 2 0.01g, MgCl 2 0.01g, KCl0.01g, FeCl 2 0.001g, CuSO 4 0.001g, MnSO 4 0.001g, ZnCl 2 0.001g, CoCl 2 0.001 g was dissolved in 1 L of deionized water, the pH was 6.0, 1% nutrient agar was added to sterilize at 121°C for 15 minutes, and then cooled to obtain a solid medium. After culturing the strain on agar solid medium at 30°C for 48 hours, colonies can be seen to grow. The colonies are round, translucent, shiny, sticky, and difficult to provoke. The strain secretes a large amount of exopolysaccharide. Scrape the colony on each 9cm plate and dissolve it with 30mL deionized water, then centrifuge at 5000×g for 20min, add 60mL ethanol-isopropanol mixture (volume ratio 1:1) to the supernatant to precipitate at room temperature, and collect the precipitate by filtra...

Embodiment 3

[0046] The cultivation of Paenibacillus edaphicus NUST16 and its application in the production of water-soluble exopolysaccharide.

[0047] Preparation medium, sucrose 50g, peptone 5g, NaH 2 PO 4 5g, CaCl 2 0.5g, MgCl 2 0.5g, KCl 0.5g, FeCl 2 0.05g, CuSO 4 0.05g, MnSO 4 0.05g, ZnCl 2 0.05g, CoCl 2 0.05 g was dissolved in 1L of deionized water, the pH was 7.0, sterilized at 121°C for 20 minutes, cooled for later use, added 1% nutrient agar, sterilized at 121°C for 15 minutes, cooled to obtain the corresponding solid medium. Pick a single colony of Paenibacillus soil NUST16 grown on the agar solid medium to the sterilized medium, culture it with shaking at 25°C for 24 hours to form a seed culture solution, and then add 7% of the seed culture solution to this spare medium Shake culture at 35°C for 48 hours to obtain fermentation broth. Add 3.5 times the volume of 95% ethanol-acetone mixture (volume ratio 1:1) to the fermentation broth, shake at room temperature, ...

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Abstract

The invention discloses soil paenibacillus and application thereof. The soil paenibacillus is soil paenibacillus NUST16, and the preservation number is CCTCC No.M2016542. According to the soil paenibacillus, the strain is unique in property, exopolysaccharide can be produced, the yield can reach 15 g/L, and the produced exopolysaccharide is easy to separate and purify and unique in property, can be directly dissolved in cold water and regulate the rheological property of an aqueous solution, has the significant effect of increasing the viscosity of the solution, can resist high-concentration Na<+>, K<+>, Ca<2+> and Mg<2+> in the solution and increase the viscosity of the solution by multiple times through simple heating-cooling treatment, is stable in property and can form thermo reversible gel.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to Paenibacillus soil and its application. Background technique [0002] Due to its special rheological properties, microbial exopolysaccharides can be used as thickeners, suspending agents or stabilizers, and are widely used in food, cosmetics and other industries. Microbial exopolysaccharides with typical rheological characteristics such as xanthan gum (Xanthan gum) can increase the viscosity of the solution, and have a certain temperature resistance. After a moderate increase in temperature, the viscosity basically remains stable; Ca 2+ It can form an irreversible brittle gel under existing conditions; thermal gel (Curdlan gum) is insoluble in water, but its suspension can form a thermally reversible elastic gel after heating to 55°C, and can form a thermally irreversible gel after heating to 80°C Brittle gel; Welan gum (Welan gum) has good heat-resistant properties and ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04C12R1/01
CPCC12P19/04C12N1/205C12R2001/01
Inventor 王世明陈向楠李晶程瑞张建法
Owner NANJING UNIV OF SCI & TECH
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