The invention relates to the field of
lactic acid bacteria starters, in particular to
lactic acid bacteria starter semi-dried particles and a preparation method thereof. The preparation method of the
lactic acid bacteria starter semi-dried particles comprises the following steps that S1, lactic acid bacteria strains are fermented and cultured expansively to obtain
fermentation broth; S2,
cell collecting is conducted on the
fermentation broth, or concentrated treatment is conducted on the
fermentation broth; S3, collected cells are dispersed into a protective agent solution again, or the concentrated
fermentation broth and the protective agent solution are mixed, and bacterine is obtained; S4, the bacterine is dripped into
liquid nitrogen to be rapidly frozen to particles, the hole
diameter of a drip mouth is 1-5 mm, and particles with the particle size being 1-5 mm are prepared; and S5, the frozen particles are subjected to vacuum
freeze drying, the
freeze drying time is controlled to be 5-15 hours, so that the
water content reaches 10-30%, and the lactic acid bacteria
starter semi-dried particles are obtained. According to the lactic acid bacteria starter semi-dried particles and the preparation method thereof, the problem of melting of
deep frozen particles is solved, and the advantages that the
deep frozen particles are high in fermentation activity and excellent in yogurt fermentation
flavor are reserved.