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60 results about "Deep frozen" patented technology

Transport container for maintaining the temperature of frozen goods

The invention relates to a transport container (1) comprising a superinsulation in the form of an evacuated insulating container (2) comprising a vacuum maintaining material (55). The transport container is provided with a cooling container (16) comprising a heat-conducting metal wool filling (57) and an organic coolant which undergoes a solid / liquid phase change in the temperature range of between −30° C. and −850° C. and has a heat of fusion of at least 50 J / ml. A slim cylindrical sample chamber (24) is used to receive deep-frozen tissue samples (26), said chamber being surrounded by the cooling container (16) and merging into a long neck opening (25) forming a single component therewith, said opening being largely filled by the insulating shaft (30) of a screwable plug (28) and sealed from the sample chamber (24). The air in the ring gap (32) created can be evacuated by means of an evacuating system (48). The plug (28) is provided with a stopper (38) protruding into the sample chamber (24) and a data logger (41) for recording the temperature in the sample chamber (24). Following the freezing of the coolant, the transport container (1) enables distribution times and intermediate storage of up to 14 days without any risk of damage to the tissue samples (26) received therein.
Owner:SIXT BERNHARD

High-density cultivation of functional probiotic lactobacillus and preparation method of deep-frozen product thereof

InactiveCN101948776AImprove survival rateMeet the requirements of fermented milk productionBacteriaMicroorganism based processesHigh densityFreeze-drying
The invention discloses high-density cultivation of functional probiotic lactobacillus and a preparation method of a deep-frozen product thereof, and relates to the cultivation of the lactobacillus and the preparation method of the deep-frozen product thereof. The invention solves the problems that the traditional lactobacillus has small viable bacteria content in the product, large dosage and bad fermentation effect in the production process and the freeze-dried preparation thereof has higher manufacturing cost and higher using cost. The cultivation comprises the steps of: (1) inoculating the aerotolerant naturalized functional probiotic lactobacillus into an enrichment medium to obtain the fermentation liquor; (2) eluting and flocculating; and (3) carrying out membrane concentration to finish the cultivation. The preparation method comprises the steps of: (1) mixing and pre-cooling a membrane concentration product and a cryoprotectant to obtain the mixture; and (2) pre-freezing the mixture, then crushing, packing and storing to obtain the finished product. The viable count of the lactobacillus in the invention is 5.4*1010 CFU/g, therefore, the dosage is decreased, the fermentation effect is good; and the deep-frozen product has low production cost, simple process and high activity and achieves the purpose of directly using.
Owner:HARBIN INST OF TECH +1

Anti-freezing structure for tunnel project in permafrost region

The invention provides an anti-freezing structure for a tunnel project in a permafrost region. The structure comprises a plurality of anti-freezing tubes and a plurality of cold and heat exchange tubes. A plurality of anti-freezing tubes are transversely arranged in a tunnel lining structure in an arch shape and are located between a preliminary support and a waterproof layer, a plurality of anti-freezing tubes are longitudinally arranged along a tunnel, and a gap is arranged between two adjacent anti-freezing tubes. The plurality of cold and heat exchange tubes are radially distributed alonga cross section of the tunnel, one end of each of the cold and heat exchange tubes communicates with one side of each of the anti-freezing tubes close to the waterproof layer, and the other end passesthrough a surrounding rock melting circle to extend into a deep frozen soil area. Both the anti-freezing tubes and the cold and heat exchange tubes are closed structures and are loaded with mediums for cold heat exchange. According to the anti-freezing structure for the tunnel project in the permafrost region, the cold and heat exchange between the deep frozen soil area and the surrounding rock melting circle can be quickly achieved, therefore, the ice melting phenomenon of a surrounding rock is improved, and the surrounding rock can be kept frozen to solve the problem of diseases in the tunnel project of the permafrost region.
Owner:CHONGQING JIAOTONG UNIVERSITY

Pipe well freeze protection device for extremely cold regions with deep frozen soil

The invention provides a pipe well freeze protection device for extremely cold regions with deep frozen soil. The pipe well freeze protection device comprises air compressors, a gas pressure tank, a water tank, a gas injection pipe, a shutoff valve, a pipe well, a lifting pipe, a submersible pump and a gas supplementing pipe. The air compressors are connected with an inlet of the gas pressure tankby the gas supplementing pipe, an outlet of the gas pressure tank is communicated with portions adjacent to the tail end of the lifting pipe via the gas injection pipe, and the shutoff valve is arranged at a joint of the lifting pipe and the water tank; a start end of the lifting pipe extends into the pipe well and is connected with the submersible pump. The pipe well freeze protection device hasthe advantages that the gas pressures of pipelines can be maintained by the gas pressure tank of the pipe well freeze protection device which is a gas injection freeze protection device, systems aresimple and stable and are low in energy consumption, and the reliability of the freeze protection systems can be improved; the pipe well freeze protection device is wide in application range, and thelifting pipe can be prevented from freezing no matter how the external temperatures change.
Owner:SOUTHWEST ELECTRIC POWER DESIGN INST OF CHINA POWER ENG CONSULTING GROUP CORP

High-density cultivation of functional bifidobacterium and preparation method of deep-frozen product thereof

The invention discloses high-density cultivation of functional bifidobacterium and a preparation method of a deep-frozen product thereof, and relates to the cultivation of the bifidobacterium and the preparation method of the deep-frozen product thereof. The invention solves the problems that the traditional bifidobacterium has small viable bacteria content in the product, large dosage and bad fermentation effect in the production process and the freeze-dried preparation thereof has higher manufacturing cost and higher using cost. The cultivation comprises the steps of: (1) inoculating the aerotolerant naturalized functional bifidobacterium into an enrichment medium to obtain the fermentation liquor; (2) eluting and flocculating; and (3) carrying out membrane concentration to finish the cultivation. The preparation comprises the steps of: (1) mixing and pre-cooling a membrane concentration product and a cryoprotectant to obtain the mixture; and (2) pre-freezing the mixture, then crushing, packing and storing to obtain the finished product. The viable count of the bifidobacterium in the invention is 5.4*1010 CFU/g, therefore, the dosage is decreased, the fermentation effect is good; and the deep-frozen product has low production cost, simple process and high activity and achieves the purpose of directly using.
Owner:HARBIN INST OF TECH +1

Lactic acid bacteria starter semi-dried particles and preparation method thereof

The invention relates to the field of lactic acid bacteria starters, in particular to lactic acid bacteria starter semi-dried particles and a preparation method thereof. The preparation method of the lactic acid bacteria starter semi-dried particles comprises the following steps that S1, lactic acid bacteria strains are fermented and cultured expansively to obtain fermentation broth; S2, cell collecting is conducted on the fermentation broth, or concentrated treatment is conducted on the fermentation broth; S3, collected cells are dispersed into a protective agent solution again, or the concentrated fermentation broth and the protective agent solution are mixed, and bacterine is obtained; S4, the bacterine is dripped into liquid nitrogen to be rapidly frozen to particles, the hole diameter of a drip mouth is 1-5 mm, and particles with the particle size being 1-5 mm are prepared; and S5, the frozen particles are subjected to vacuum freeze drying, the freeze drying time is controlled to be 5-15 hours, so that the water content reaches 10-30%, and the lactic acid bacteria starter semi-dried particles are obtained. According to the lactic acid bacteria starter semi-dried particles and the preparation method thereof, the problem of melting of deep frozen particles is solved, and the advantages that the deep frozen particles are high in fermentation activity and excellent in yogurt fermentation flavor are reserved.
Owner:JIANGSU WECARE BIOTECHNOLOGY CO LTD
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