Lactic acid bacteria starter semi-dried particles and preparation method thereof

Abstract

The invention relates to the field of lactic acid bacteria starters, in particular to lactic acid bacteria starter semi-dried particles and a preparation method thereof. The preparation method of the lactic acid bacteria starter semi-dried particles comprises the following steps that S1, lactic acid bacteria strains are fermented and cultured expansively to obtain fermentation broth; S2, cell collecting is conducted on the fermentation broth, or concentrated treatment is conducted on the fermentation broth; S3, collected cells are dispersed into a protective agent solution again, or the concentrated fermentation broth and the protective agent solution are mixed, and bacterine is obtained; S4, the bacterine is dripped into liquid nitrogen to be rapidly frozen to particles, the hole diameter of a drip mouth is 1-5 mm, and particles with the particle size being 1-5 mm are prepared; and S5, the frozen particles are subjected to vacuum freeze drying, the freeze drying time is controlled to be 5-15 hours, so that the water content reaches 10-30%, and the lactic acid bacteria starter semi-dried particles are obtained. According to the lactic acid bacteria starter semi-dried particles and the preparation method thereof, the problem of melting of deep frozen particles is solved, and the advantages that the deep frozen particles are high in fermentation activity and excellent in yogurt fermentation flavor are reserved.

Description

technical field [0001] The invention relates to the field of lactic acid bacteria starters, in particular to semi-dry granules of lactic acid bacteria starters and a preparation method thereof. Background technique [0002] At present, due to the beneficial effects of lactic acid bacteria on human and animal health, it has been widely used, such as as a starter for human food, beverages, animal feed, etc., or as a starter for human food, health products, animal food and feed, etc. additive. [0003] The lactic acid bacteria starter in the prior art roughly has two kinds of product forms, and a kind of is the powder or granule (below moisture 5% or 10%) after vacuum freeze-drying, and another kind is liquid nitrogen deep-frozen granulation and Undried deep-frozen granules (moisture content is generally 60-80%). Both have their own advantages and disadvantages. Among them, liquid nitrogen deep-frozen granulation is better than ordinary freeze-dried powder due to its low dama...

AI Technical Summary

Problems solved by technology

Both have their own advantages and disadvantages. Among them, liquid nitrogen deep-frozen granulation is better than ordinary freeze-dried powder due to its low damage, regular spherical particle product appearance, and good water solubility due to the rapid freezing of liquid nitrogen.

[0004] Although the starter after vacuum freeze-drying is more convenient to use and store, it is only necessary to mix different freeze-dried single strains according to the formula ratio when preparing formula starter products, but the bacteria will be affected during the drying process. Damage, fermentation activity will be reduced

[0005] Liquid nitrogen deep-frozen granular starter, because it has not gone through the drying process, has high fermentation activity and lower production costs than dried starter products; however, the deep-frozen granular starter must be strictly in a low-temperature environment, otherwise the frozen particles will melt. Cannot be stored again, or refrozen, which brings a series of inconveniences

For example, when producing deep-frozen granule starter products containing different strains, you cannot simply mix them like freeze-dried starter, otherwise the deep-frozen granule single strain will melt, so, or in the process of preparing the bacteria slurry, the single strain Mixing, or mixing deep-frozen granules in a low-temperature environment and using special equipment; moreover, deep-frozen granule starter products require a cold chain throughout the process, and when the temperature is higher than -5°C, they will melt and affect the survival of cells The rate and the use of the product have greatly affected the popularization and application of the deep-frozen granular starter

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