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Endogenetic polymexa bacillus of plant for prophyiaxis and promoting growth and application thereof

A technology of Paenibacillus polymyxa and endogenous bacteria in plants, which is applied in the direction of bacteria, can solve the problems of endogenous Paenibacillus polymyxa that has no effect on disease prevention and growth promotion, and achieve outstanding control effects, increase yield, and increase fresh weight. Effect

Inactive Publication Date: 2005-10-26
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic research on Paenibacillus polymyxa is mostly focused on combined nitrogen fixation, and there is no report on endogenous Paenibacillus polymyxa that has the effect of disease prevention and growth promotion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Liquid preparation

[0039] The plant endogenous Paenibacillus polymyxa Jaas cd strain is inoculated on the slant of a test tube, the medium is NA, and cultured at a constant temperature of 25° C. for 2-4 days to obtain a test tube species.

[0040] The test tube seed was inoculated into NB medium, and after 28°C constant temperature shaking culture overnight, it was transferred to NB medium according to the inoculum amount of 0.1%, and the culture solution after 28°C constant temperature shaking culture for 14 hours could be the preparation. It can also be transferred to a fermenter in which the fermentation medium is a bean cake powder medium for fermentation and production.

[0041] Formula one, common bacteria NA culture medium (weight %)

[0042] 0.3% beef extract, 0.1% yeast extract,

[0043] Peptone 0.5%, Glucose 1.0%,

[0044]Agar 1.5-2.0%, pH 6.8-7.0.

[0045] Formula two, common bacteria NB culture medium (weight %)

[0046] 0.3% beef extract, 0...

Embodiment 2

[0051] Example 2 Cotton production increase test

[0052] Under the premise of the same cotton variety, density, fertilizer dosage, and cultivation management measures, two treatments were set up: root irrigation with diluted Jaas cd bacterial solution and root irrigation with water, randomized block design, each treatment was repeated 4 times, and the plot area Not less than 20m 2 . The time for root irrigation and medication is July 13 and July 22 respectively. On September 10, the number of big bells, small bells and flocculation was investigated. The results show that the root irrigation with the plant endogenous Paenibacillus polymyxa bacterium solution adopted in the present invention increases the production by 8.22% compared with the control group with water irrigation.

Embodiment 3

[0053] Example 3 Growth-promoting effect on cotton seedlings

[0054] The germinated cotton seeds were sown in pots in the greenhouse, and the diluted Jaas cd bacterial solution was poured on the cotton seeds and pot soil at the same time. After 60 days, the height of the seedlings was measured and the fresh weight was weighed. The test results showed that the strain had no effect on cotton seedlings. It has obvious growth-promoting effect, and the plant height increases by 29.9% compared with the control, and the fresh weight increases by 45.2% compared with the control.

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Abstract

The present invention relates to Paenibacillus polymuxa and its applications, which is from a kind of prevented disease and spurred development plant. It belongs to prevented and controlled disease technology fields of crop. The Jaas cd can enter, propagate and transmit into the body of this plant on the condition that the lower chroma of bacterium, meanwhile, it can promote growth, increase output and prevent disease, especially to prevent passes disease. This Jass cd is widely used in reagent, the price is cheap, manipulation is simple, it does good to prevent field zoology environment, reduce rudimental prison, it suits to Chinese yielding requirement of non-social effects of pollution farm produce.

Description

Technical field: [0001] The invention relates to a plant endogenous Paenibacillus polymyxa for disease prevention and growth promotion and its application in the field of biological control, belonging to the technical field of crop disease prevention and control. Background technique: [0002] Plant endophytes are normal microorganisms that can live in plants without causing obvious symptoms. These microorganisms can colonize the intercellular space or intracellularity of plants, and establish a harmonious and united relationship with the host plant. Since the beginning of the last century, people have begun to study endophytic bacteria. In recent years, research results have shown that plant endophytic bacteria not only use host plants as their habitats, but also have growth-promoting, disease-preventing, and nitrogen-fixing effects on host plants. Biological effects, and endophytic bacteria are also good carriers for introducing exogenous genes into plants. Therefore, pl...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 林玲顾本康夏正俊
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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