High-temperature-resistant phytase activity estimation method

Abstract

The invention is applicable to the technical field of feed stuff and processing technology, provides a high-temperature-resistant phytase activity estimation method and solves the problem that the influence of various links in practical production environments on high-temperature-resistant phytase is not taken into full consideration by a current phytase measuring method. The method includes the steps: firstly, sufficiently mixing feed samples with the high-temperature-resistant phytase, placing mixture into a high-pressure sterilization pot, performing reaction for 3 minutes at the temperature of 75-90 DEG C, rapidly taking out an aluminum-plastic bag after reaction, and rapidly cooling the aluminum-plastic bag in ice-water mixture; secondly, simulating digestion in an in-vitro microenvironment; finally, measuring phosphorus by a colorimetric method to obtain the content of water-soluble phosphorus released by the feed samples with phytase functions in simulated gastric environments, and activity of the high-temperature-resistant phytase is estimated. By simulating high-temperature and high-humidity granulation environments of granules and the quantity of water-soluble phosphorus multiply released by substrates in the gastric environments, in-vitro practical action effects of the high-temperature-resistant phytase after simulation of the granulation process are judged.

Description

technical field [0001] The invention relates to the technical field of feed raw materials and processing technology, in particular to a method for evaluating the activity of high-temperature-resistant phytase. Background technique [0002] Phosphorus plays an important role in the nutrition of livestock and poultry. Animals must obtain enough phosphorus from feed to maintain normal life activities and achieve better production performance. The total phosphorus content in plant feed is very high, but most of it exists in the form of phytate phosphorus, which is difficult to be digested and utilized by livestock and poultry. Phytase can release the phosphorus of phytate phosphorus in plant feed, thereby reducing the amount of exogenous phosphorus. usage, save cost, and reduce environmental pollution. In addition, since phytic acid often exists in the form of complexes with other nutrients (such as zinc, iron, etc.), and is not easy to dissociate, the use of phytase can improv...

AI Technical Summary

Problems solved by technology

[0004] At present, the detection methods of high temperature resistant phytase mostly use water bath method, dry heat method or damp heat method to treat high temperature resistant phytase, and then use GB / T 18634—2009 "Determination of Feed Phytase Activity - Spectroscopic The photometric method is used to test the enzyme activity, but the conditions set in the early sample processing cannot completely simulate the actual granulation conditions on the spot, the results are quite different, and the actual reference value is low, and the phytase activity test in the later stage does not consider The loss of feed under pepsin and strong acid environment has no realistic guiding significance to the amount of addition in the feed formula. The present invention solves the above problems, fully considering the impact of each link in the actual production environment on the high temperature resistant phytase. Effect, a comprehensive evaluation of the effective activity of thermostable phytase in animals

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