High-temperature-resistant phytase activity estimation method

A high-temperature-resistant phytase and phytase technology is applied in the field of feed raw materials and processing technology, which can solve the problems of lack of feed formula addition, large differences in results, and inability to completely simulate pelleting conditions, and achieve accurate test data and high accuracy. The effect of reducing errors

Inactive Publication Date: 2017-02-22
沈阳波音饲料有限公司
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Problems solved by technology

[0004] At present, the detection methods of high temperature resistant phytase mostly use water bath method, dry heat method or damp heat method to treat high temperature resistant phytase, and then use GB/T 18634—2009 "Determination of Feed Phytase Activity - Spectroscopic The photometric method is used to test the enzyme activity, but the conditions set in the early sample processing cannot completely simulate the actual granulation conditions on the spot, the results are quite different, and the ac

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  • High-temperature-resistant phytase activity estimation method

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Embodiment 1

[0042] A method for evaluating high temperature resistant phytase activity, specifically comprising the following steps:

[0043] 1. Determination of the actual water content of the feed sample, see GB / T 6435-2006 for the specific determination method.

[0044] 2. High temperature resistant phytase high temperature treatment:

[0045](1) Weigh 5g (accurate to 0.0001g) (m) of feed samples respectively and place them in aluminum-plastic bags as the treatment group and blank control group. The feed samples are composed of the following components according to mass percentage: corn 60% , soybean meal 20%, cotton meal 10%, DDGS 10%.

[0046] (2) Add the high-temperature-resistant phytase in one of the aluminum-plastic bags according to the enzyme activity addition of 2,000FYT / kg, that is, the enzyme activity of 2mg is the high-temperature-resistant phytase (accurate to 0.1) of 5,000FYT / g mg) was added into the aluminum-plastic bag (the blank group was not added), and the two were...

Embodiment 2

[0060] A method for evaluating high temperature resistant phytase activity, specifically comprising the following steps:

[0061] 1. Determination of the actual water content of the feed sample, see GB / T 6435-2006 for the specific determination method.

[0062] 2. High temperature resistant phytase high temperature treatment:

[0063] (1) Weigh 3g (accurate to 0.0001g) (m) of feed samples respectively, place them in aluminum-plastic bags, and serve as treatment group and blank control group respectively. The feed samples are composed of the following components according to mass percentage: corn 60%, soybean meal 20%, cotton meal 10% and DDGS 10%.

[0064] (2) Add the high-temperature-resistant phytase in one of the aluminum-plastic bags according to the enzyme activity addition of 2,000FYT / kg, that is, the enzyme activity of 0.6mg is the high-temperature-resistant phytase (accurate to 0.1) of 10000FYT / g mg) was added into the aluminum-plastic bag (the blank group was not ad...

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Abstract

The invention is applicable to the technical field of feed stuff and processing technology, provides a high-temperature-resistant phytase activity estimation method and solves the problem that the influence of various links in practical production environments on high-temperature-resistant phytase is not taken into full consideration by a current phytase measuring method. The method includes the steps: firstly, sufficiently mixing feed samples with the high-temperature-resistant phytase, placing mixture into a high-pressure sterilization pot, performing reaction for 3 minutes at the temperature of 75-90 DEG C, rapidly taking out an aluminum-plastic bag after reaction, and rapidly cooling the aluminum-plastic bag in ice-water mixture; secondly, simulating digestion in an in-vitro microenvironment; finally, measuring phosphorus by a colorimetric method to obtain the content of water-soluble phosphorus released by the feed samples with phytase functions in simulated gastric environments, and activity of the high-temperature-resistant phytase is estimated. By simulating high-temperature and high-humidity granulation environments of granules and the quantity of water-soluble phosphorus multiply released by substrates in the gastric environments, in-vitro practical action effects of the high-temperature-resistant phytase after simulation of the granulation process are judged.

Description

technical field [0001] The invention relates to the technical field of feed raw materials and processing technology, in particular to a method for evaluating the activity of high-temperature-resistant phytase. Background technique [0002] Phosphorus plays an important role in the nutrition of livestock and poultry. Animals must obtain enough phosphorus from feed to maintain normal life activities and achieve better production performance. The total phosphorus content in plant feed is very high, but most of it exists in the form of phytate phosphorus, which is difficult to be digested and utilized by livestock and poultry. Phytase can release the phosphorus of phytate phosphorus in plant feed, thereby reducing the amount of exogenous phosphorus. usage, save cost, and reduce environmental pollution. In addition, since phytic acid often exists in the form of complexes with other nutrients (such as zinc, iron, etc.), and is not easy to dissociate, the use of phytase can improv...

Claims

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Application Information

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IPC IPC(8): C12Q1/44
CPCC12Q1/44
Inventor 刘娇王昕陟李东全杨光郭伟王密李忠秋孙丹彤
Owner 沈阳波音饲料有限公司
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