Microbial preparation for improving freshwater aquaculture water body and preparation method thereof

A microbial preparation and water body improvement technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost and complicated preparation, and achieve the effect of low cost, simple preparation method and few types

Inactive Publication Date: 2013-11-20
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Although microbial products have been used in aquaculture, there are still problems such as complicated preparation and high cost, and new microbial preparations for aquaculture water improvement are needed.

Method used

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  • Microbial preparation for improving freshwater aquaculture water body and preparation method thereof
  • Microbial preparation for improving freshwater aquaculture water body and preparation method thereof
  • Microbial preparation for improving freshwater aquaculture water body and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Raw material composition:

[0051]

[0052] Preparation:

[0053] step 1:

[0054] Bacillus subtilis and Bacillus licheniformis were respectively inoculated in the first culture medium under aseptic conditions, 30 DEG C-35 DEG C, single-species cultivation 48 hours, obtain Bacillus subtilis and Bacillus licheniformis seed culture liquid, its The number of live bacteria is greater than 5.0×10 9 cfu / ml. Among them, the first medium is composed of molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium chloride 0.2-0.25wt%, iron sulfate 30 -35wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt% (including vitamin H2-2.3wt%, vitamin B12-15wt% aqueous solution) and the rest of water, the pH value is 6.5-6.8 .

[0055] Inoculate Rhodopseudomonas palustris in the second culture medium under aseptic conditions, under the conditions of 4500-10000lx light intensity and 29°C-33°C temperature, culture for 6-8 days ...

Embodiment 2

[0066] Raw material composition:

[0067]

[0068] Preparation:

[0069] step 1:

[0070] According to the method described in Example 1, the seed culture liquid of Bacillus subtilis, the seed culture liquid of Bacillus licheniformis and the seed culture liquid of Rhodopseudomonas palustris were provided.

[0071] Step 2,

[0072] According to the method described in Example 1, a high-concentration concentrated bacterial solution was obtained as a mother solution.

[0073] Step 3,

[0074] Move the concentrated bacterial liquid into the matrix composed of zeolite powder + calcium carbonate for feed + cornstarch + molasses and mix. The mixed fluid is granulated on a swing machine, and then boiled and dried. The mass percentage of moisture after drying is 5-7%.

[0075] Wherein, based on the total weight of the matrix (carrier and nutrients), the matrix is ​​composed of 75wt% zeolite powder, 10wt% calcium carbonate, 8wt% cornstarch, and 7wt% molasses.

[0076] Step 4,

...

Embodiment 3

[0079] Raw material composition:

[0080]

[0081] Preparation:

[0082] step 1:

[0083] According to the method described in Example 1, the seed culture liquid of Bacillus subtilis, the seed culture liquid of Bacillus licheniformis and the seed culture liquid of Rhodopseudomonas palustris were provided.

[0084] Step 2,

[0085] According to the method described in Example 1, a high-concentration concentrated bacterial solution was obtained as a mother solution.

[0086] Step 3,

[0087] Move the concentrated bacterial liquid into the matrix composed of zeolite powder + calcium carbonate for feed + cornstarch + molasses and mix. The mixed fluid is granulated on a swing machine, and then boiled and dried. The mass percentage of moisture after drying is 5-7%.

[0088] Wherein, based on the total weight of the matrix (carrier and nutrients), the matrix is ​​composed of 65wt% zeolite powder, 15wt% calcium carbonate, 5wt% cornstarch and 5wt% molasses.

[0089] Step 4,

[0...

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Abstract

The invention provides a microbial preparation for improving freshwater aquaculture water body. The microbial preparation comprises the following raw materials by weight percentage: 25-30 wt% of bacillus subtilis, 25-30 wt% of bacillus licheniformis and 15-20 wt% of rhodopseudomonas palustris. The invention also provides a method for preparing the microbial preparation, which comprises the following steps of: sequentially inoculating the seed culture liquids of the bacillus subtilis, the bacillus licheniformis and the rhodopseudomonas palustris into a culture medium, fermenting to obtain a bacteria liquid, and mixing the obtained bacteria liquid with a carrier and nutrient substances to obtain the microbial preparation. The microbial preparation provided by the invention can significantly improve the water quality of the aquaculture water body and can also improve the meat quality of bred animals.

Description

technical field [0001] The invention relates to the technical field of water body improvement, in particular to a microbial preparation which can be used for freshwater aquaculture water body improvement and a preparation method thereof. Background technique [0002] China is the world's largest aquaculture country. According to statistics, China's inland aquaculture water area is 27.34 million hectares, seawater aquaculture area is 4.38 million hectares, and rice field fish farming area is 1.52 million hectares in 2001. Especially in the water towns in southern China, aquaculture plays an important role in agricultural production. Many areas regard aquaculture as an important way for local farmers to get rich. Aquaculture has gradually become one of the pillar industries in aquaculture agriculture. [0003] At the same time, China is also one of the countries with the most serious pollution of aquaculture water. Since ancient times, there has been a saying in China that "r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12R1/07C12R1/125C12R1/01
Inventor 江瀚陈仁爱
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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