Mixed microbial preparation for aquaculture and preparation method thereof

A technology of mixed microorganisms and aquaculture, which is applied in the field of mixed microorganism preparations for aquaculture and its preparation, which can solve problems such as deterioration of water bodies, achieve the effects of reducing usage, improving quality and quantity, and simple and easy preparation methods

Active Publication Date: 2014-03-26
广东天之源农业科技有限公司
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Problems solved by technology

[0003] The main technical problem to be solved by the present invention is to provide a mixed microbial preparation for aquaculture and its preparation method, which c

Method used

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Embodiment 1

[0020] (1) Strain expansion: 30g Bacillus subtilis, 25g Bacillus licheniformis, 20g Bacillus megaterium, 20g Rhodopseudomonas palustris, 40g brewer’s yeast, 20g photosynthetic bacteria, 13g nitrifying bacteria, 3g Streptococcus faecalis, 5g Bacillus natto and 10g bifidobacteria were inoculated into a medium with an electric stirrer under aseptic conditions. The components of the culture medium in the medium were: peptone 5g, yeast extract 3g, corn flour 10g, glucose 10g, maltose 10g, NaCl 3g, FeCl 0.002g, ammonium chloride 2g, KH 2 PO 4 1g, MgSO 4 ·7H 2 0 0.5g, 800 mL of water, pH 7.0, sterilized at 150°C for 45 min; the inoculation amount is 5-8% of the total amount of the culture solution, at a constant temperature of 40°C, at 250-300 revolutions / min Stir at the rotating speed, and at the same time use the oxygen generating pump to add 0.5~1m to the culture medium 3 The gas flow rate per hour is filled with oxygen. After 48 hours of fermentation, the viable counts of Bacillus...

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Abstract

The invention discloses a mixed microbial preparation for aquaculture and a preparation method thereof. The mixed microbial preparation comprises the following bacteria by weight: 30 to 35 parts of Bacillus subtilis, 20 to 30 parts of Bacillus licheniformis, 20 to 25 parts of Bacillus megaterium, 15 to 20 parts of Rhodopseudomonas palustris, 30 to 50 parts of beer yeast, 18 to 20 parts of photosynthetic bacteria, 10 to 13 parts of nitrobacteria, 1 to 5 parts of Streptococcus faecalis, 5 to 10 parts of Bacillus natto and 10 to 15 parts of Bifidobacterium. The mixed microbial preparation is prepared through enlarged cultivation, normal temperature separation, low temperature separation, low temperature drying and the like. The preparation method in the invention has the advantages of easiness, easy practicability and capacity of realizing batch production; the prepared mixed microbial preparation can significantly reduce the content of ammonia nitrogen and nitrite in a water body, inhibit propagation of harmful bacteria, improve water quality and disease resistance of fishes and shrimps, lower down consumption of drugs and antibiotics and effectively improve the quality and the quantity of aquaculture products.

Description

Technical field [0001] The invention relates to the technical field of aquaculture, in particular to a mixed microbial preparation for aquaculture and a preparation method thereof. Background technique [0002] In recent years, with the rapid development of the aquaculture industry, the high density and intensification of aquaculture has continued to increase, and the supply of aquatic products has also increased rapidly. However, this kind of high-density and intensive aquaculture has not only brought serious pollution to the water environment, but has also gradually reduced the quality of aquatic products, and the development of aquaculture has been subject to certain restrictions. This is mainly due to the following factors: First, in intensive aquaculture, the concentration of fish, shrimp, and crabs is high, and a large amount of excreted waste is hoarded at the bottom of the water body. These excreted wastes contain a large amount of organic matter, which consumes a lot of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C02F3/34C12R1/125C12R1/10C12R1/11C12R1/865C12R1/46C12R1/07C12R1/01
Inventor 杜伟林
Owner 广东天之源农业科技有限公司
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