Household garbage deodorizing and sterilizing composite inoculant and preparation method thereof

A technology of compound bacterial agent and household garbage, which is applied in the field of environmental protection, can solve the problems of accelerating the aerobic decomposition of garbage, and achieve the effect of eliminating odor, odor gas and odor

A technology of compound bacterial agent and household garbage, which is applied in the field of environmental protection, can solve the problems of accelerating the aerobic decomposition of garbage, and achieve the effect of eliminating odor, odor gas and odor

CN103992968AActive Publication Date: 2014-08-20ANHUI QINGMING ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Household garbage deodorizing and sterilizing composite inoculant and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Step 1: preparing a compound culture solution of brewer's yeast, Lactobacillus plantarum, Bacillus thuringiensis and Rhodopseudomonas paluscans.

[0044] Inoculate brewer's yeast, Bacillus thuringiensis, Lactobacillus plantarum and Rhodopseudomonas palustris activated on the agar slant into the liquid seed medium of the triangular flask after high temperature sterilization at 121°C (brewer's yeast: PDA medium, at 28°C Bacillus thuringiensis: YG medium, at 30°C, cultivate at 180rpm for 72h; Lactobacillus plantarum: MRS medium, at 28°C, culture in sealed static state for 48h; Rhodopseudomonas palustris: Under the light of 3000lx, at 30°C, sealed static culture for 72h, medium formula: sodium acetate 0.5 wt%, sodium chloride 0.1 wt%, ammonium sulfate 0.03 wt%, potassium dihydrogen phosphate 0.05 wt%, dipotassium hydrogen phosphate 0.04 wt%, magnesium sulfate 0.04 wt%, calcium chloride 0.005 wt%, manganese sulfate 0.05 wt%, ferrous sulfate 0.0005 wt%, yeast extract 0.05 wt%...

Embodiment 2

[0050] Step 1: preparing a compound culture solution of brewer's yeast, Lactobacillus plantarum, Bacillus thuringiensis and Rhodopseudomonas paluscans.

[0051]Inoculate brewer's yeast, Bacillus thuringiensis, Lactobacillus plantarum and Rhodopseudomonas palustris activated on the agar slant into the liquid seed medium of the triangular flask after high temperature sterilization at 121°C (brewer's yeast: PDA medium, at 28°C Bacillus thuringiensis: YG medium, at 30°C, cultivate at 180rpm for 72h; Lactobacillus plantarum: MRS medium, at 28°C, culture in sealed static state for 48h; Rhodopseudomonas palustris: Under the light of 3000lx, at 30°C, sealed static culture for 72h, medium formula: sodium acetate 0.5 wt%, sodium chloride 0.1 wt%, ammonium sulfate 0.03 wt%, potassium dihydrogen phosphate 0.05 wt%, dipotassium hydrogen phosphate 0.04 wt%, magnesium sulfate 0.04 wt%, calcium chloride 0.005 wt%, manganese sulfate 0.05 wt%, ferrous sulfate 0.0005 wt%, yeast extract 0.05 wt%,...

Embodiment 3

[0057] Step 1: preparing a compound culture solution of brewer's yeast, Lactobacillus plantarum, Bacillus thuringiensis and Rhodopseudomonas paluscans.

[0058] Inoculate brewer's yeast, Bacillus thuringiensis, Lactobacillus plantarum and Rhodopseudomonas palustris activated on the agar slant into the liquid seed medium of the triangular flask after high temperature sterilization at 121°C (brewer's yeast: PDA medium, at 28°C Bacillus thuringiensis: YG medium, at 30°C, cultivate at 180rpm for 72h; Lactobacillus plantarum: MRS medium, at 28°C, culture in sealed static state for 48h; Rhodopseudomonas palustris: Under the light of 3000lx, at 30°C, sealed static culture for 72h, medium formula: sodium acetate 0.5 wt%, sodium chloride 0.1 wt%, ammonium sulfate 0.03 wt%, potassium dihydrogen phosphate 0.05 wt%, dipotassium hydrogen phosphate 0.04 wt%, magnesium sulfate 0.04 wt%, calcium chloride 0.005 wt%, manganese sulfate 0.05 wt%, ferrous sulfate 0.0005 wt%, yeast extract 0.05 wt%...

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Abstract

The invention belongs to the technical field of environment protection, and especially relates to a composite microbial preparation for garbage deodorizing and sterilization and a preparation method thereof. The microbial preparation comprises beauveria bassiana, saccharomyces cerevisiae, lactobacillus plantar, rhodopseudomonas palustris, and bacillus thruingiensis. The comprehensive composite inoculant provided by the invention can remove the garbage odor and kill insects such as mosquito, fly, cockroach, and the like; and can be used in locations such as rubbish transfer station, digestion tank, public lavatory, landfill, etc. to remove odors and kill insects. The stinky odors given off by the garbage can be rapidly degraded under the cooperation of five microorganisms, furthermore, the bacterium proliferation is inhibited, the insects in the garbage piles such as mosquito, fly, cockroach, and the like can be killed, and the microecological environment of garbage piles can be improved, so the composite inoculant has a very important economic and environment-protection meaning.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a compound microbial preparation for deodorizing and killing garbage and a preparation method thereof. Background technique [0002] With the acceleration of my country's urbanization process and the increase in population, the generation of urban domestic waste and the environmental pollution it has caused people to pay attention to Issues such as the emission of bad odor, the breeding of mosquitoes and flies, and the spread of diseases and insect pests have already had a certain impact on the quality of life of residents and the local ecological environment. If the management of municipal solid waste collection and transfer is not standardized, it will seriously damage the ecology of the city and cause people's psychological unhappiness. [0003] At present, the following problems exist in the municipal solid waste treatment process: [0004] (1) T...

Claims

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Application Information

Patent Timeline
20 Aug 2014
Publication
CN103992968A
IPC
C12N1/20; C12N1/18; C12N1/14; B09B3/00; C12R1/645; C12R1/865; C12R1/25; C12R1/01; C12R1/07
Inventors
赵海泉; 胡子全