Preparation technique of photosynthetic bacteria preparation

Abstract

The invention discloses a preparation technique of a photosynthetic bacteria reagent, which comprises rhodopseudomonas palustris strain cultivation steps that include activated strain cultivation with an inclined plane, strain cultivation with a shaking table, cultivation with an aerated fermenting jar and the like, and adopts the culture medium comprising a basic culture medium and a growth promoting agent. The basic culture medium comprises following components by mass: 0.9 to 1.1 percent of glucose, 0.04 to 0.06 percent of carbamide, 0.02 to 0.03 percent of dipotassium hydrogen phosphate, 0.02 to 0.03 percent of monopotassium phosphate, 0.02 to 0.03 percent of magnesium sulfate, 0.20 to 0.30 percent of sodium chloride, 0.03 to 0.05 percent of calcium chloride and 98.40 to 98.77 percent of water. The added growth promoting agent is 0.10 to 0.30 percent of fulvic acid by mass referring to the basic culture medium. The photosynthetic bacteria reagent that is obtained by the preparation technique can reach the lysate concentration of 4000 to 6000 million per milliliter, and can be used in multiple fields of aquiculture, wastewater processing, crop production and the like.

Description

Technical field: [0001] The invention belongs to the field of biological technology, and relates to a preparation process of photosynthetic bacteria preparation. Background technique: [0002] Photosynthetic bacteria are a group of bacteria that can use organic matter as oxygen provider and carbon source under anaerobic light or aerobic dark conditions. Photosynthetic bacteria are widely used in aquaculture, wastewater treatment, planting and other fields. At present, a variety of methods and devices for cultivating photosynthetic bacteria have been developed. These methods are mainly based on the principle that photosynthetic bacteria can grow under anaerobic light conditions. The facilities are usually glass containers, plastic film bags, etc., and the facilities are simple. , the investment is small, and the existing problems are mainly that the cultivation time is long (generally 4-7 days), the production efficiency is low, the bacterial content of the culture solution ...

AI Technical Summary

Problems solved by technology

At present, a variety of methods and devices for cultivating photosynthetic bacteria have been developed. These methods are mainly based on the principle that photosynthetic bacteria can grow under anaerobic light conditions. The facilities are usually glass containers, plastic film bags, etc., and the facilities are simple. , the investment is small, and the existing problems are mainly that the cultivation time is long (generally 4-7 days), the production efficiency is low, the bacterial content of the culture solution is small (generally only 3 billion / mL), and it is easy to be contaminated. Unstable quality

Although some people have carried out the test of cultivating photosynthetic bacteria with aerated fermentation, it is still difficult to standardize, scale and industrialize the production of photosynthetic bacteria preparations due to the lack of perfect technology such as medium formula and culture process conditions.